Molecular Human Reproduction, Vol. 6, No. 7, 618-626,
July 2000
© 2000 European Society of Human Reproduction and Embryology
Uterine physiology |
Ovarian hormones modulate monocyte chemotactic protein-1 expression in endometrial cells of women with endometriosis
1 Department of Obstetrics and Gynecology and 2 Department of Medicine, Faculty of Medicine, Laval University, Quebec, Canada
Abstract
Endometriosis, a frequent oestrogen-dependent disease believed to result from an aberrant proliferation of endometrial tissue outside the uterine cavity, is associated with an increased expression of monocyte chemotactic protein-1 (MCP-1) in the intrauterine endometrium. This makes it plausible that migrating endometrial cells are intrinsically able to initiate monocyte chemoattraction and activation, a phenomenon which has been consistently observed in the peritoneal cavity of patients and recently in their eutopic endometrium. To elucidate the mechanisms involved in the regulation of MCP-1 expression in eutopic endometrial cells, we studied the effects of ovarian hormones and found that oestradiol (10–9 and 10–8 mol/l) markedly increased MCP-1 mRNA steady-state levels and protein secretion by endometrial cells in response to interleukin-1ß (IL-1ß) (0.1 ng/ml). The IL-1ß-induced MCP-1 expression was even higher following pretreatment of cells with both oestradiol (10–9 mol/l) and progesterone (5x10–8 mol/l). This did not seem to be due to increased MCP-1 mRNA stability, but rather to a higher level of gene transcription. Our results provide evidence that ovarian steroids regulate, indirectly, the synthesis and the secretion of a potent chemotactic and activating factor for monocytes/macrophages by endometrial cells of women with endometriosis and reveal a new mechanism for oestradiol action.
endometriosis/endometrium/oestradiol/MCP-1
Notes
3 To whom correspondence should be addressed at: Laboratoire d'Endocrinologie de la Reproduction, Centre de Recherche, Hôpital Saint-Franciois d'Assise, 10 rue de l'Espinay, Local D0-711, Québec, Canada, G1L 3L5. E-mail: ali.akoum{at}crsfa.ulaval.ca
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