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Molecular Human Reproduction, Vol. 6, No. 8, 707-711, August 2000
© 2000 European Society of Human Reproduction and Embryology

Embryo development

Transcription of homeobox-containing genes detected in cDNA libraries derived from human unfertilized oocytes and preimplantation embryos

James Adjaye1 and Marilyn Monk

Molecular Medicine Unit, Molecular Embryology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK

Abstract

Genes containing the evolutionarily conserved homeodomain sequence encode a family of DNA-binding transcription factors whose functions are crucial for embryonic development in vertebrates, invertebrates and plants. We describe the detection and analysis of transcripts of homeobox-containing genes present in cDNA libraries generated from human unfertilized oocytes, single cleavage stage embryos (2-cell, 4-cell, 8-cell and blastocyst) and a 10-week old whole fetus. Using degenerate primers derived from sequences within helix 1 and helix 3 of the highly conserved region of the Antennapedia-class homeodomain, a 166 bp band was detected in all the cDNA libraries tested. Subcloning of the oocyte-derived band revealed that it contained a heterogeneous group of 166 bp fragments. Sequence analysis of 40 independent clones demonstrated the presence of HOXA7, HOXD8, and HOXD1 sequences, the ubiquitously expressed POU family member, OCT1, and HEX, a homeotic gene expressed in haematopoietic cells.

embryonic cDNA libraries/homeobox gene expression/human preimplantation development/maternal mRNA/OCT-4

Notes

1 To whom correspondence should be addressed at: Molecular Medicine Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK. E-mail: J.Adjaye{at}ich.ucl.ac.uk


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