Molecular Human Reproduction, Vol. 6, No. 9, 779-788,
September 2000
© 2000 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Developmental expression of Y-box protein 1 mRNA and alternatively spliced Y-box protein 3 mRNAs in spermatogenic cells in mice
Department of Biology, University of Massachusetts Boston, 100 Morrissey Boulevard, Boston, MA 02125–3393, USA
Abstract
Y-box proteins bind DNA and RNA and are characterized by a cold shock domain and a carboxyl-terminus containing clusters of aromatic and basic residues that alternate with clusters of acidic residues. Y-box proteins 1 and 3 in mouse testis were cloned here by 3' rapid amplification of cDNA ends (RACE) using a degenerate primer. Northern blots and reverse transcription–polymerase chain reaction (RT–PCR) established that the levels of Y-box protein 1 and 3 mRNAs are regulated individually: (i) Y-box protein 1 mRNA is strongly expressed in kidney, whereas Y-box protein 3 mRNA is strongly expressed in heart and muscle; (ii) Y-box protein 1 and 3 mRNAs are weakly expressed in early prepubertal testis and strongly expressed in pachytene spermatocytes, round spermatids, and elongated spermatids; and (iii) prepubertal testes and meiotic and haploid spermatogenic cells express two alternatively spliced Y-box protein 3 mRNAs encoding isoforms with different carboxyl termini, whereas somatic tissues primarily express one form. Sucrose gradients reveal that ~27% of both Y-box protein 3 mRNAs are translationally active in adult testis. In conclusion, spermatogenic cells in mice express five isoforms of Y-box proteins including Y-box protein 1, and two isoforms each of Y-box proteins 2 and 3. This multiplicity is intriguing because Y-box proteins are thought to activate transcription and repress translation in spermatogenic cells.
cold-shock domain/spermatogenesis/transcriptional regulation/translational regulation/Y-box proteins
Notes
1 To whom correspondence should be addressed at: Department of Biology, University of Massachusetts Boston100 Morrissey Boulevard, Boston, MA 02125–3393, USA. E-mail:kenneth.kleene{at}umb.edu
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