Molecular Human Reproduction, Vol. 6, No. 9, 801-809,
September 2000
© 2000 European Society of Human Reproduction and Embryology
Embryo development |
Detection of human novel developmental genes in cDNA derived from replicate individual preimplantation embryos
1 Molecular Embryology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, and 2 Assisted Conception Unit, Academic Department of Obstetrics and Gynaecology, Kings College School of Medicine and Dentistry, Denmark Hill, London, SE5 9RS, UK
Abstract
We have constructed amplified cDNA preparations from replicate samples of human oocytes and individual preimplantation embryos. Differential display of the cDNA preparations shows disparate patterns of gene expression in the individual embryos at all stages of preimplantation development. The variation in patterns of genes expressed is in part due to the low starting cell number undergoing the reverse transcription–polymerase chain reaction (RT–PCR) step in the preparation of the amplified cDNAs. Despite this variability, the use of replicate embryo samples makes it possible to identify and isolate human genes specifically expressed at the different stages of human preimplantation development from the unfertilized oocyte to the blastocyst stage.
cDNA libraries/differential display/developmental genes/human/preimplantation embryo
Notes
3 To whom correspondence should be addressed at: Molecular Embryology Unit, 30 Guilford Street, London WC1 1EH, UK. E-mail: mmonk{at}ich.ucl.ac.uk
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