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Molecular Human Reproduction, Vol. 6, No. 9, 829-834, September 2000
© 2000 European Society of Human Reproduction and Embryology

Uterine physiology

Increased concentrations of secretory leukocyte protease inhibitor in peritoneal fluid of women with endometriosis

Koichiro Shimoya1,2,3, Akihiro Moriyama1, Isao Ogata1, Toshikatu Nobunaga1, Masayasu Koyama1, Chihiro Azuma1 and Yuji Murata1

1 Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 2-2 Yamada-oka, Suita City, Osaka 565-0871, and 2 Department of Obstetrics and Gynecology, Osaka Police Hospital, 10–31 Kitayama-cho, Tennouji-ku, Osaka 543-8502, Japan

Abstract

Secretory leukocyte protease inhibitor (SLPI) is a potent inhibitor of human leukocyte elastase. We investigated whether SLPI was present in the peritoneal fluid of women with endometriosis and to clarify the role of SLPI in the pathogenesis of endometriosis. Western blot analyses revealed that SLPI protein was detected as a 12 kDa band in peritoneal fluid. The peritoneal fluid concentrations of SLPI, elastase and interleukin-6 were assayed by enzyme-linked immunosorbent assays (ELISA). SLPI concentrations and the SLPI/elastase ratio in the peritoneal fluid of women with endometriosis were higher than in samples from women without endometriosis. There was no significant correlation between concentrations of SLPI and interleukin-6 in the peritoneal fluid. Immunohistochemistry using an anti-SLPI polyclonal antibody revealed positive staining in peritoneal macrophages, but not lymphocytes. The present findings suggest that SLPI found in the peritoneal fluid of patients with endometriosis may contribute to the pathogenesis of endometriosis.

elastase/endometriosis/peritoneal fluid/peritoneal macrophage/secretory leukocyte protease inhibitor (SLPI)

Notes

3 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 2–2 Yamada-oka, Suita City, Osaka 565-0871, Japan. E-mail: shimoya{at}gyne.med.osaka-u.ac.jp


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