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Molecular Human Reproduction, Vol. 6, No. 9, 849-854, September 2000
© 2000 European Society of Human Reproduction and Embryology

Genetic diagnosis

Improvements of preimplantation diagnosis of aneuploidy by using microwave hybridization, cell recycling and monocolour labelling of probes

M. Bahçe3, T. Escudero1, M. Sandalinas1, L. Morrison2, M. Legator2 and S. Munné1,4

1 Institute for Reproductive Medicine and Science, Saint Barnabas Medical Center, Livingston, NJ, 2 Vysis Inc, Downers Grove, IL, USA and 3 Department of Medical Genetics Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey

Abstract

Recent studies indicate that preimplantation genetic diagnosis of aneuploidy significantly reduces spontaneous abortions and increases pregnancy rates in women of advanced maternal age undergoing in-vitro fertilization. A new protocol was developed involving cell recycling (sequential hybridizations) and microwave hybridization of repetitive probes in order to obtain in a few hours enumeration results for chromosomes X, Y, 1, 13, 15, 16, 18, 21 and 22 in single blastomeres. The error rate using this protocol was significantly lower than in previous protocols and the analysis of nine chromosomes can be achieved in a shorter time than before.

monosomy/mosaicism/pregnancy loss/spontaneous abortions/trisomy

Notes

4 To whom correspondence should be addressed at: Saint Barnabas Medical Center, 101 Old Short Hills Rd, Suite 501, Livingston, NJ-07052, USA. E-mail: santi.munne{at}embryos.net


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