Molecular Human Reproduction, Vol. 7, No. 1, 21-26,
January 2001
© 2001 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Presence of antibodies to sperm YLP12 synthetic peptide in sera and seminal plasma of immunoinfertile men
Division of Research, Department of Obstetrics and Gynecology, Medical College of Ohio, Toledo, OH 43614, USA
Abstract
Using an enzyme-linked immunosorbent assay (ELISA), sera (n = 15) and seminal plasma (n = 30) from antisperm antibody-positive immunoinfertile men (n = 45) and from fertile men (n = 45), were tested for the immunoreactivity with the synthetic YLP12 sperm peptide. Of the 15 immunoinfertile sera tested, 46% were positive for immunoglobulin (Ig)M, 73% for IgG, and 40% for IgA. Of the 30 samples of immunoinfertile seminal plasma tested, 10% were positive for IgM, 20% for IgG, and 43% for IgA. None of the sera or seminal plasma from fertile men showed a positive reaction. There was no significant correlation between the sperm immobilization technique (SIT) or tray agglutination technique (TAT) titres or percentage binding in immunobead binding technique (IBT) and the antibody reactivity for any class in the ELISA. The YLP12 peptide conjugated to bovine serum albumin–Sepharose 4B beads pulled out IgG antibodies from the serum of the immunoinfertile, but not the fertile, men. The beads pulled out IgA antibodies from the immunoinfertile, but not the fertile, seminal plasma. The immuno-affinity purified antipeptide antibodies reacted with a specific band of 72 ± 5 kDa in the human testis and with a specific band of ~50 ± 5 kDa in the human sperm extracts. The YLP12 peptide may have applications in the specific diagnosis and treatment of male infertility and in contraceptive vaccine development.
antisperm antibodies/fertilization/infertility/semen/sperm antigens
Notes
1 To whom correspondence should be addressed at: Division of Research, Health Education Building, Room 211, Medical College of Ohio, 3055 Arlington Avenue, Toledo, Ohio, 43614–5806, USA. E-mail: Rnaz{at}mco.edu
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