Molecular Human Reproduction, Vol. 7, No. 1, 49-55,
January 2001
© 2001 European Society of Human Reproduction and Embryology
Ovary and oogenesis |
Association between spindle assembly checkpoint expression and maternal age in human oocytes
1 Gamete and Embryo Research Laboratory, Institute for Reproductive Medicine and Science of Saint Barnabas, West Orange, NJ 07052, 2 Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, and 3 Department of Biological Sciences, Florida International University, Miami, FL 33199, USA
Abstract
The spindle assembly checkpoint modulates the timing of anaphase initiation in response to the improper alignment of chromosomes at the metaphase plate. If defects are detected, a signal is transduced to halt further progression of the cell cycle until correct bipolar attachment to the spindle is achieved. The mitotic arrest deficient (MAD2) and budding uninhibited by benomyl (BUB1) genes encode conserved kinetochore-associated proteins believed to be components of the checkpoint regulatory pathway. A failure in this surveillance system could lead to genomic instability that may underlie the increased incidence of aneuploidy in the gametes of older women. To explore this possibility, the concentrations of these transcripts in human oocytes at various stages of maturation were determined by real-time rapid cycle fluorescent reverse transcription–polymerase chain reaction (RT–PCR). The results obtained following quantitative analysis suggest that these messages degrade as oocytes age. Potentially, this may impair checkpoint function in older oocytes and may be a contributing factor in age-related aneuploidy.
BUBI gene/MAD2 gene/oocyte/RT-PCR/spindle assembly checkpoint
Notes
4 To whom correspondence should be addressed at: Gamete and Embryo Research Laboratory, Institute for Reproductive Medicine and Science of Saint Barnabas, West Orange, New Jersey, 07052, USA. E-mail: nury.steuerwald{at}embryos.net
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. T. Jones Meiosis in oocytes: predisposition to aneuploidy and its increased incidence with age Hum. Reprod. Update, March 1, 2008; 14(2): 143 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tatone, F. Amicarelli, M. C. Carbone, P. Monteleone, D. Caserta, R. Marci, P. G. Artini, P. Piomboni, and R. Focarelli Cellular and molecular aspects of ovarian follicle ageing Hum. Reprod. Update, March 1, 2008; 14(2): 131 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cukurcam, I. Betzendahl, G. Michel, E. Vogt, C. Hegele-Hartung, B. Lindenthal, and U. Eichenlaub-Ritter Influence of follicular fluid meiosis-activating sterol on aneuploidy rate and precocious chromatid segregation in aged mouse oocytes Hum. Reprod., March 1, 2007; 22(3): 815 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Assou, T. Anahory, V. Pantesco, T. Le Carrour, F. Pellestor, B. Klein, L. Reyftmann, H. Dechaud, J. De Vos, and S. Hamamah The human cumulus-oocyte complex gene-expression profile Hum. Reprod., July 1, 2006; 21(7): 1705 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vialard, C. Petit, M. Bergere, D. M. Gomes, V. Martel-Petit, R. Lombroso, Y. Ville, H. Gerard, and J. Selva Evidence of a high proportion of premature unbalanced separation of sister chromatids in the first polar bodies of women of advanced age Hum. Reprod., May 1, 2006; 21(5): 1172 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Tatone, M. C. Carbone, R. Gallo, S. Delle Monache, M. Di Cola, E. Alesse, and F. Amicarelli Age-Associated Changes in Mouse Oocytes During Postovulatory In Vitro Culture: Possible Role for Meiotic Kinases and Survival Factor BCL2 Biol Reprod, February 1, 2006; 74(2): 395 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Delimitreva, R. Zhivkova, E. Isachenko, N. Umland, and P.L. Nayudu Meiotic abnormalities in in vitro-matured marmoset monkey (Callithrix jacchus) oocytes: development of a non-human primate model to investigate causal factors Hum. Reprod., January 1, 2006; 21(1): 240 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T Jones Mammalian egg activation: from Ca2+ spiking to cell cycle progression Reproduction, December 1, 2005; 130(6): 813 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Steuerwald, M. D. Steuerwald, and J. B. Mailhes Post-ovulatory aging of mouse oocytes leads to decreased MAD2 transcripts and increased frequencies of premature centromere separation and anaphase Mol. Hum. Reprod., September 1, 2005; 11(9): 623 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Homer, A. McDougall, M. Levasseur, A. P. Murdoch, and M. Herbert RNA interference in meiosis I human oocytes: towards an understanding of human aneuploidy Mol. Hum. Reprod., June 1, 2005; 11(6): 397 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ma, D. Zhang, Y. Hou, Y.-H. Li, Q.-Y. Sun, X.-F. Sun, and W.-H. Wang Reduced Expression of MAD2, BCL2, and MAP Kinase Activity in Pig Oocytes after In Vitro Aging Are Associated with Defects in Sister Chromatid Segregation During Meiosis II and Embryo Fragmentation After Activation Biol Reprod, February 1, 2005; 72(2): 373 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Homer, A. McDougall, M. Levasseur, K. Yallop, A. P. Murdoch, and M. Herbert Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes Genes & Dev., January 15, 2005; 19(2): 202 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sandalinas, C. Marquez, and S. Munne Spectral karyotyping of fresh, non-inseminated oocytes Mol. Hum. Reprod., June 1, 2002; 8(6): 580 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Sun, H. Yin, and U. Eichenlaub-Ritter Differential chromosome behaviour in mammalian oocytes exposed to the tranquilizer diazepam in vitro Mutagenesis, September 1, 2001; 16(5): 407 - 417. [Abstract] [Full Text] [PDF]
|
|