Molecular Human Reproduction, Vol. 7, No. 1, 49-55,
January 2001
© 2001 European Society of Human Reproduction and Embryology
Ovary and oogenesis |
Association between spindle assembly checkpoint expression and maternal age in human oocytes
1 Gamete and Embryo Research Laboratory, Institute for Reproductive Medicine and Science of Saint Barnabas, West Orange, NJ 07052, 2 Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, and 3 Department of Biological Sciences, Florida International University, Miami, FL 33199, USA
Abstract
The spindle assembly checkpoint modulates the timing of anaphase initiation in response to the improper alignment of chromosomes at the metaphase plate. If defects are detected, a signal is transduced to halt further progression of the cell cycle until correct bipolar attachment to the spindle is achieved. The mitotic arrest deficient (MAD2) and budding uninhibited by benomyl (BUB1) genes encode conserved kinetochore-associated proteins believed to be components of the checkpoint regulatory pathway. A failure in this surveillance system could lead to genomic instability that may underlie the increased incidence of aneuploidy in the gametes of older women. To explore this possibility, the concentrations of these transcripts in human oocytes at various stages of maturation were determined by real-time rapid cycle fluorescent reverse transcription–polymerase chain reaction (RT–PCR). The results obtained following quantitative analysis suggest that these messages degrade as oocytes age. Potentially, this may impair checkpoint function in older oocytes and may be a contributing factor in age-related aneuploidy.
BUBI gene/MAD2 gene/oocyte/RT-PCR/spindle assembly checkpoint
Notes
4 To whom correspondence should be addressed at: Gamete and Embryo Research Laboratory, Institute for Reproductive Medicine and Science of Saint Barnabas, West Orange, New Jersey, 07052, USA. E-mail: nury.steuerwald{at}embryos.net
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