Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Schmid, C.
Right arrow Articles by Krawetz, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmid, C.
Right arrow Articles by Krawetz, S. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 7, No. 10, 903-911, October 2001
© 2001 European Society of Human Reproduction and Embryology

Testis and spermatogenesis

Sperm nuclear matrix association of the PRM1->PRM2->TNP2 domain is independent of Alu methylation

Carl Schmid1, Henry H.Q. Heng2,3, Carol Rubin1, Christine J. Ye4 and Stephen A. Krawetz2,5,6

1 Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, 2 Center for Molecular Medicine and Genetics, 3 Department of Pathology and the Karmanos Cancer Institute, Wayne State University School of Medicine 4 SeeDNA Biotech Inc., Windsor, Ontario, Canada, 5 Department of Obstetrics and Gynecology and the Institute for Scientific Computing, Wayne State University, Detroit, MI 48201, USA

Abstract

Genes or multigenic chromosomal regions are organized by the nuclear matrix into a series of functionally discrete genic domains. Biophysical analysis of the human chromosome 16p13.13 region has shown that the PRM1->PRM2->TNP2 protamine containing multigenic locus is bounded by two sperm nuclear matrix attachment regions (MAR). This domain exists in a transcriptionally readied or potentiated (i.e. open) chromatin state when associated with the nuclear matrix. The MAR-bounded PRM1->PRM2->TNP2 locus is nestled in an Alu repetitive element dense region. Fluorescence in-situ hybridization, analysis of sperm nuclear matrix/halo preparations showed that the PRM1->PRM2->TNP2 domain specifically localizes to the sperm nuclear matrix. This raised the question of whether nuclear matrix association and gene expression in this locus is mediated by Alu methylation. The methylation status of the various Alu elements contained within the human PRM1->PRM2->TNP2 locus was therefore assayed. The seven Alu elements tested, including those associated with the matrix attachment regions within the PRM1->PRM2->TNP2 locus, were fully methylated in sperm DNA. Conversely, these same Alu repeats were hypomethylated within the erythroleukaemic cell line, K562, which does not express any of the genes from this domain. This study shows that Alu methylation status is independent of attachment of PRM1->PRM2->TNP2 locus to the nuclear matrix and that Alu methylation does not play a leading role in the regulation of this domain.

Alu repeat/methylation/protamine/nuclear matrix/spermatozoa

Notes

6 To whom correspondence should be addressed at: Department of Obstetrics & Gynecology, Center for Molecular Medicine & Genetics, Institute for Scientific Computing, Wayne State University School of Medicine, 253 C.S.Mott Center, 275 E. Hancock, Detroit, MI 48201, USA. E-mail: steve{at}compbio.med.wayne.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
T. Guastafierro, B. Cecchinelli, M. Zampieri, A. Reale, G. Riggio, O. Sthandier, G. Zupi, L. Calabrese, and P. Caiafa
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery
J. Biol. Chem., August 8, 2008; 283(32): 21873 - 21880.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. H. Q. Heng, S. Goetze, C. J. Ye, G. Liu, J. B. Stevens, S. W. Bremer, S. M. Wykes, J. Bode, and S. A. Krawetz
Chromatin loops are selectively anchored using scaffold/matrix-attachment regions
J. Cell Sci., March 1, 2004; 117(7): 999 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. C. Ostermeier, Z. Liu, R. P. Martins, R. R. Bharadwaj, J. Ellis, S. Draghici, and S. A. Krawetz
Nuclear matrix association of the human {beta}-globin locus utilizing a novel approach to quantitative real-time PCR
Nucleic Acids Res., June 15, 2003; 31(12): 3257 - 3266.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S. Repping, J. W.A. de Vries, S. K.M. van Daalen, C. M. Korver, N. J. Leschot, and F. van der Veen
The use of spermHALO-FISH to determine DAZ gene copy number
Mol. Hum. Reprod., April 1, 2003; 9(4): 183 - 188.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.