Molecular Human Reproduction, Vol. 7, No. 10, 935-945,
October 2001
© 2001 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Epididymal epithelium immortalized by simian virus 40 large T antigen: a model to study epididymal gene expression
1 IHF, Institute for Hormone and Fertility Research at the University, Hamburg, Germany and 2 Department of Reproductive Sciences and Medicine, Imperial College of Science, Technology, and Medicine, Hammersmith Hospital, London, UK
Abstract
Primary cultures of the differentiated, adult epididymal duct epithelium were immortalized by retroviral transduction with the simian virus (SV)40 large T antigen. The canine epididymis was chosen here as a model with high human relevance, representing a convenient and acceptable source of differentiated epididymal tissue and, compared to other animal models, expressing a relatively large number of gene products which are also expressed by the human epididymis. To determine whether the immortalized canine epididymal (IMCE) cells retained a phenotype comparable to the original tissue, epithelial cytokeratins, various epididymal transcription factors as well as mRNAs encoding abundant epididymal secretory proteins, were studied as molecular markers. All IMCE populations obtained after transduction were of epithelial origin. The nuclear androgen receptor (AR) and the polyoma enhancer activator (PEA3), as well as the epididymal mRNA encoding the canine counterparts of human HE1, HE4 and HE5/CD52 epididymal mRNA, were retained in all populations tested. The majority of tested clones were oestrogen receptor ER
-positive, but ERß-negative, while one ER-negative cell population was positive for ERß. The IMCE populations described thus represent useful permanent tools for studying gene expression of the epididymal duct epithelium, and for other types of experiments, examples including drug effects and toxicity on the epididymis.
epididymis/HE1/HE4/immortalization/transcription factors
Notes
3 To whom correspondence should be addressed at: IHF Institut für Hormon- und Fortpflanzungsforschung, Grandweg 64, D-22529 Hamburg. E-mail: kirchhoff{at}ihf.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Dufresne, N. St-Pierre, R. S. Viger, L. Hermo, and D. G. Cyr Characterization of a Novel Rat Epididymal Cell Line to Study Epididymal Function Endocrinology, November 1, 2005; 146(11): 4710 - 4720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Seenundun and B. Robaire Cloning and Characterization of the 5{alpha}-Reductase Type 2 Promoter in the Rat Epididymis Biol Reprod, April 1, 2005; 72(4): 851 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oyhenart, J.L. Dacheux, F. Dacheux, B. Jegou, and N. Raich Expression, Regulation, and Immunolocalization of Putative Homeodomain Transcription Factor 1 (PHTF1) in Rodent Epididymis: Evidence for a Novel Form Resulting from Proteolytic Cleavage Biol Reprod, January 1, 2005; 72(1): 50 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Kirby, L. Yang, J. C. Labus, R. J. Lye, N. Hsia, R. Day, G. A. Cornwall, and B. T. Hinton Characterization of Epididymal Epithelial Cell-Specific Gene Promoters by In Vivo Electroporation Biol Reprod, August 1, 2004; 71(2): 613 - 619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sipila, R. Shariatmadari, I. T. Huhtaniemi, and M. Poutanen Immortalization of Epididymal Epithelium in Transgenic Mice Expressing Simian Virus 40 T Antigen: Characterization of Cell Lines and Regulation of the Polyoma Enhancer Activator 3 Endocrinology, January 1, 2004; 145(1): 437 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Araki, K. Suzuki, R. J. Matusik, M. Obinata, and M.-C. Orgebin-Crist Immortalized Epididymal Cell Lines From Transgenic Mice Overexpressing Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene J Androl, November 1, 2002; 23(6): 854 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kirchhoff The dog as a model to study human epididymal function at a molecular level Mol. Hum. Reprod., August 1, 2002; 8(8): 695 - 701. [Abstract] [Full Text] [PDF]
|
|