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Molecular Human Reproduction, Vol. 7, No. 10, 979-985, October 2001
© 2001 European Society of Human Reproduction and Embryology

Implantation and pregnancy

Nitric oxide increases matrix metalloproteinase-1 production in human uterine cervical fibroblast cells

Masahiro Yoshida1, Norimasa Sagawa1,4, Hiroaki Itoh1, Shigeo Yura1, Daizo Korita1, Kazuyo Kakui1, Naoyoshi Hirota2, Takashi Sato3, Akira Ito3 and Shingo Fujii1

1 Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, 2 Pharmacology Group, R&D Administration and Coordination, Research & Development Division, Nippon Organon K.K., 1-5-90 Tomobuchi-cho, Miyakojima-ku, Osaka 534-0016 and 3 Department of Biochemistry, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-0392, Japan

Abstract

Since uterine cervical ripening is an active biochemical process similar in part to an inflammatory reaction, nitric oxide (NO) has been proposed as a key mediator of this event. However, the mechanism by which NO modulates human cervical ripening has not been fully elucidated. In the present study we investigated the presence of NO synthases in human uterine cervix by immunohistochemistry and reverse transcriptase–polymerase chain reaction analysis. Furthermore, we examined the presence of NO-mediated regulation of matrix metalloproteinase-1 (MMP-1) production in cultured human uterine cervical fibroblast cells using enzyme-linked immunosorbent assay and Northern blot analysis. Endothelial and inducible NO synthases were detected in the form of mRNA and protein expression in pregnant uterine cervix. Interleukin-1{alpha} (IL-1{alpha}) increased the expression of inducible NO synthase mRNA in cultured human uterine cervical fibroblast cells. IL-1{alpha} also increased MMP-1 secretion from the cultured cervical fibroblast cells. This IL-1{alpha}-augmented MMP-1 secretion was partially but significantly blocked by an NO synthase inhibitor, NG-nitro-L-arginine methyl ester. On the other hand, NO donors increased MMP-1 production in the cultured cervical fibroblast cells. These findings together suggest that NO contributes to the process of cervical ripening via enhancement of MMP-1 production, and that IL-1{alpha} increases MMP-1 secretion from cervical fibroblasts at least in part via NO synthesis.

interleukin-1{alpha}/matrix metalloproteinases/nitric oxide/nitric oxide synthase/uterine cervical ripening

Notes

4 To whom correspondence should be addressed. E-mail: fetus{at}kuhp.kyoto-u.ac.jp


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