Molecular Human Reproduction, Vol. 7, No. 11, 1015-1022,
November 2001
© 2001 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men
1 Departments of Urology, 2 Physiology, 3 Obstetrics and Gynecology and 4 Institute of Molecular Medicine, National Cheng Kung University, College of Medicine, Tainan, 5 Taiwan United Birth Promoting Experts, Tainan and 6 Department of Early Childhood Education and Nursery, Chia Nan University of Pharmacy and Science, Tainan, Taiwan
Abstract
The DAZ (Deleted in AZoospermia) gene cluster on the Y chromosome is a strong candidate for the azoospermia factor. The DAZ gene was derived from an autosomal homologue, DAZL (DAZ-Like). This study was designed to assess the functional role of DAZL in human spermatogenesis. The expression patterns and mRNA transcript levels of DAZL in the testes of 17 azoospermic men were therefore examined by immunohistochemical staining and quantitative competitive reverse transcription–polymerase chain reaction. DAZL protein was expressed in the cytoplasm of primary spermatocytes and weakly in spermatogonia. It was detected in the testicular tissues of all subjects with germ cells present. The copy number of the DAZL transcript in normal spermatogenesis (n = 4), hypospermatogenesis or maturation arrest (n = 6), and Sertoli cell-only syndrome (n = 7) ranged from 1.22x106 to 1.63x106 per ng of RNA, 1.19x105 to 2.82x105 per ng of RNA and 2.83x104 to 1.23x105 per ng of RNA respectively. DAZL transcripts were lower in men with spermatogenic failure, and a significant difference was found between the three groups (P < 0.0001). This study suggests that DAZL may play an important role in the human spermatogenic processes of both mitosis and meiosis.
azoospermia/DAZL/spermatogenesis/testis
Notes
7 To whom correspondence should be addressed at: Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, 138 Sheng-Li Road, Tainan, Taiwan 704. E-mail: paolink{at}mail.ncku.edu.tw
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