Molecular Human Reproduction, Vol. 7, No. 2, 163-168,
February 2001
© 2001 European Society of Human Reproduction and Embryology
Uterine physiology |
Chemokine bioactivity of RANTES in endometriotic and normal endometrial stromal cells and peritoneal fluid
1 Department of Obstetrics and Gynecology, 72076 Tübingen, Germany, 2 Center for Reproductive Sciences, University of California, San Francisco, CA 94143, USA and 3 Department of Obstetrics and Gynecology, 48149 Münster, Germany
Abstract
Endometriotic lesions secrete chemokines that recruit immune cells into the peritoneal cavity. The accumulation of these immune cells, especially activated macrophages and T lymphocytes, is thought to mediate inflammatory symptoms associated with endometriosis. Previous studies have demonstrated that RANTES (regulated on activation, normal T cell expressed and secreted) is synthesized by endometriotic stromal cells and circulates in peritoneal fluid, commensurate with the stage of endometriosis. In the current studies, we used the human monocytic cell line, U937, to assay chemotactic activity in cell culture conditioned media and peritoneal fluid from patients with endometriosis and normal controls. We demonstrated expression of the human RANTES receptors CCR-1 and CCR-5 in U937 cells and peritoneal macrophages. Over a range of 0–1000 pg/ml recombinant human RANTES had a direct, linear effect on monocyte migration. Conditioned media and peritoneal fluid induced dose-dependent effects on monocyte migration that were correlated with concentrations of immunoreactive RANTES (as measured by enzyme-linked immunosorbent assay) and the severity of endometriosis. Heat denaturation of the RANTES protein or addition of anti-human RANTES antibodies neutralized the chemoattractant effects of conditioned media and peritoneal fluid. RANTES stimulation of monocyte recruitment may be an important pathogenetic target for the treatment of infertility and pain associated with endometriosis.
chemokine/endometriosis/endometrium/peritoneal fluid/RANTES
Notes
4 To whom correspondence should be addressed. E-mail: rtaylor{at}socrates.ucsf.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Barbonetti, M.R.C. Vassallo, F. Pelliccione, A. D'Angeli, R. Santucci, B. Muciaccia, M. Stefanini, F. Francavilla, and S. Francavilla Beta-chemokine receptor CCR5 in human spermatozoa and its relationship with seminal parameters Hum. Reprod., December 1, 2009; 24(12): 2979 - 2987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wieser, J. Yu, J. Park, A. Gaeddert, M. Cohen, J.-L. Vigne, and R. N. Taylor A Botanical Extract from Channel Flow Inhibits Cell Proliferation, Induces Apoptosis, and Suppresses CCL5 in Human Endometriotic Stromal Cells Biol Reprod, August 1, 2009; 81(2): 371 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Weiss, L. T. Goldsmith, R. N. Taylor, D. Bellet, and H. S. Taylor Inflammation in Reproductive Disorders Reproductive Sciences, February 1, 2009; 16(2): 216 - 229. [Abstract] [PDF]
|
|
|
|
|
|
L. Fraccaroli, J. Alfieri, L. Larocca, M. Calafat, G. Mor, C. P. Leiros, and R. Ramhorst A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation Hum. Reprod., January 1, 2009; 24(1): 166 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Agic, S. Djalali, M. M. Wolfler, G. Halis, K. Diedrich, and D. Hornung Combination of CCR1 mRNA, MCP1, and CA125 Measurements in Peripheral Blood as a Diagnostic Test for Endometriosis Reproductive Sciences, November 1, 2008; 15(9): 906 - 911. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Barbonetti, M.R.C. Vassallo, C. Antonangelo, V. Nuccetelli, A. D'Angeli, F. Pelliccione, M. Giorgi, F. Francavilla, and S. Francavilla RANTES and human sperm fertilizing ability: effect on acrosome reaction and sperm/oocyte fusion Mol. Hum. Reprod., July 1, 2008; 14(7): 387 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yu, Y. Wang, W.-H. Zhou, L. Wang, Y.-Y. He, and D.-J. Li Combination of estrogen and dioxin is involved in the pathogenesis of endometriosis by promoting chemokine secretion and invasion of endometrial stromal cells Hum. Reprod., July 1, 2008; 23(7): 1614 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Finas, M. Huszar, A. Agic, S. Dogan, H. Kiefel, S. Riedle, D. Gast, R. Marcovich, F. Noack, P. Altevogt, et al. L1 cell adhesion molecule (L1CAM) as a pathogenetic factor in endometriosis Hum. Reprod., May 1, 2008; 23(5): 1053 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Styer, B. T. Sullivan, M. Puder, D. Arsenault, J. C. Petrozza, T. Serikawa, S. Chang, T. Hasan, R. R. Gonzalez, and B. R. Rueda Ablation of Leptin Signaling Disrupts the Establishment, Development, and Maintenance of Endometriosis-Like Lesions in a Murine Model Endocrinology, February 1, 2008; 149(2): 506 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Wren, Y. Wu, and S.-W. Guo A system-wide analysis of differentially expressed genes in ectopic and eutopic endometrium Hum. Reprod., August 1, 2007; 22(8): 2093 - 2102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Naz and R. Sellamuthu Receptors in Spermatozoa: Are They Real? J Androl, September 1, 2006; 27(5): 627 - 636. [Full Text] [PDF]
|
|
|
|
|
|
D. Uygur, H. Aytan, S. Zergeroglu, and S. Batioglu Leflunomide--an Immunomodulator--Induces Regression of Endometrial Explants in a Rat Model of Endometriosis Reproductive Sciences, July 1, 2006; 13(5): 378 - 383. [Abstract] [PDF]
|
|
|
|
 |
|
 F. Wieser, J.-L. Vigne, I. Ryan, D. Hornung, S. Djalali, and R. N. Taylor Sulindac Suppresses Nuclear Factor-{kappa}B Activation and RANTES Gene and Protein Expression in Endometrial Stromal Cells from Women with Endometriosis J. Clin. Endocrinol. Metab., December 1, 2005; 90(12): 6441 - 6447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Januchowski, A. K. Breborowicz, H. Ofori, P. Jedrzejczak, L. Pawelczyk, and P. P. Jagodzinski Detection of a Short CCR5 Messenger RNA Isoform in Human Spermatozoa J Androl, September 1, 2004; 25(5): 757 - 760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Lebovic, V. A. Chao, and R. N. Taylor Peritoneal Macrophages Induce RANTES (Regulated on Activation, Normal T Cell Expressed and Secreted) Chemokine Gene Transcription in Endometrial Stromal Cells J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1397 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Antinolo, R.M. Fernandez, J.A. Noval, J.L. Molini, and S. Borrego Analysis of the involvement of CCR5-{Delta}32 and CCR2-V64I variants in the development of endometriosis Mol. Hum. Reprod., March 1, 2004; 10(3): 155 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhao, E. A. Pritts, V. A. Chao, J.-F. Savouret, and R. N. Taylor Dioxin stimulates RANTES expression in an in-vitro model of endometriosis Mol. Hum. Reprod., September 1, 2002; 8(9): 849 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhao, D. I. Lebovic, and R. N. Taylor Long-Term Progestin Treatment Inhibits RANTES (Regulated on Activation, Normal T Cell Expressed and Secreted) Gene Expression in Human Endometrial Stromal Cells J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2514 - 2519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Isobe, H. Minoura, K. Tanaka, T. Shibahara, N. Hayashi, and N. Toyoda The effect of RANTES on human sperm chemotaxis Hum. Reprod., June 1, 2002; 17(6): 1441 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Pritts, D. Zhao, E. Ricke, L. Waite, and R. N. Taylor PPAR-{gamma} Decreases Endometrial Stromal Cell Transcription and Translation of RANTES in Vitro J. Clin. Endocrinol. Metab., April 1, 2002; 87(4): 1841 - 1844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Lebovic, V. A. Chao, J.-F. Martini, and R. N. Taylor IL-1{beta} Induction of RANTES (Regulated upon Activation, Normal T Cell Expressed and Secreted) Chemokine Gene Expression in Endometriotic Stromal Cells Depends on a Nuclear Factor-{kappa}B Site in the Proximal Promoter J. Clin. Endocrinol. Metab., October 1, 2001; 86(10): 4759 - 4764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hornung, L. L. Waite, E. A. Ricke, F. Bentzien, D. Wallwiener, and R. N. Taylor Nuclear Peroxisome Proliferator-Activated Receptors {{alpha}} and {{gamma}} Have Opposing Effects on Monocyte Chemotaxis in Endometriosis J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3108 - 3114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Vigne, D. Hornung, M. D. Mueller, and R. N. Taylor Purification and Characterization of an Immunomodulatory Endometrial Protein, Glycodelin J. Biol. Chem., May 11, 2001; 276(20): 17101 - 17105. [Abstract] [Full Text] [PDF]
|
|