Molecular Human Reproduction, Vol. 7, No. 3, 245-253,
March 2001
© 2001 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Calcitonin, angiotensin II and FPP significantly modulate mouse sperm function*
1 Endocrinology and Reproduction Research Group, School of Biomedical Sciences, King's College London, Guy's Campus, London Bridge, London SE1 1UL, 2 Histopathology, St George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, and 3 Biomedical Sciences, Queen Mary Westfield College, Mile End Road, London E1 4NS, UK
Abstract
Fertilization-promoting peptide (FPP) regulates the adenylyl cyclase (AC)/cAMP pathway to elicit capacitation-dependent responses, stimulating capacitation in uncapacitated spermatozoa and then arresting it in capacitated cells, thereby inhibiting spontaneous acrosome reactions. Like FPP, calcitonin and angiotensin II are found in seminal plasma and so might affect sperm function; this study investigated responses in uncapacitated and capacitated mouse spermatozoa to these three peptides. Both calcitonin (5 ng/ml) and angiotensin II (1 and 10nmol/l), like FPP (100nmol/l), significantly stimulated capacitation, assessed using chlortetracycline (CTC) fluorescence and fertilization in vitro analyses. Combinations of two or three peptides, at high and low, non-stimulatory concentrations, were more stimulatory than the individual peptides, suggesting that they may act on the same signalling pathway, plausibly AC/cAMP; preliminary data indicate that calcitonin does stimulate cAMP production. In capacitated cells, FPP and calcitonin elicited pertussis toxin-sensitive inhibition of spontaneous acrosome loss, suggesting involvement of inhibitory G proteins; angiotensin II had no detectable effect. When all three peptides were used, angiotensin II did not interfere with inhibitory responses to FPP/calcitonin. These results suggest that angiotensin II, calcitonin and FPP may somehow modulate the AC/cAMP signal transduction pathway, but the precise mechanisms involved have yet to be elucidated.
acrosome reaction/cAMP/adenylyl cyclase/capacitation/fertilization-promoting peptide
Notes
* This study has previously been published in part, see Fraser et al. (1999).
4 To whom correspondence should be addressed. E-mail: lynn.fraser{at}kcl.ac.uk
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