Molecular Human Reproduction, Vol. 7, No. 4, 319-323,
April 2001
© 2001 European Society of Human Reproduction and Embryology
Gonadotrophins inhibit and activin induces expression of inhibin/activin ßB subunit mRNA in cultured human granulosa-luteal cells
1 Department of Pathology, P.O.Box 21, University of Helsinki, FIN-00014 Helsinki, 2 Department of Pediatrics, P.O.Box 1777, Kuopio University Hospital, FIN-70211 Kuopio and 3 Department of Obstetrics and Gynecology, Helsinki University Central Hospital, FIN-00290 Helsinki, Finland
During the human menstrual cycle, serum inhibin concentrations fluctuate in a cyclic fashion. To examine the regulation of inhibin/activin ßB subunit gene expression in ovarian granulosa–luteal cells, the levels of ßB subunit mRNA were determined in primary cultures of human granulosa–luteal cells treated with gonadotrophins and protein kinase modulators. Granulosa cells were obtained from women undergoing an IVF programme. The cells were enzymatically dispersed, separated from red blood cells, and maintained in culture for 5–10 days before addition of different agents. Northern blot analysis with specific oligonucleotide probes was performed to study inhibin/activin ßB subunit mRNA levels. Both LH and FSH reduced the accumulation of ßB subunit mRNA in a dose-dependent manner. The protein kinase A activator, (Bu)2cAMP, and the protein kinase inhibitor staurosporine also inhibited ßB subunit mRNA expression dose-dependently. Activin A increased dose-dependently ßB subunit mRNA expression. Our study suggests that activin-induced and gonadotrophin-inhibited ßB subunit expression in granulosa cells might be key factors in the transition from inhibin B to inhibin A dominance during the menstrual cycle.
activin/cAMP/gonadotrophin/granulosa cells/inhibin
4 To whom correspondence should be addressed at: Department of Pathology, P.O.Box 21, University of Helsinki, FIN-00014 Helsinki, Finland. E-mail: Jiangi.Liu{at}helsinki.fi
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