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Molecular Human Reproduction, Vol. 7, No. 6, 505-512, June 2001
© 2001 European Society of Human Reproduction and Embryology

Testis and spermatogenesis

MUC1 in normal and impaired spermatogenesis

Folker E. Franke1,5, Sigurd Kraus2, Claus Eiermann2, Katharina Pauls1, El-Nasir Lalani4 and Martin Bergmann3

1 Departments of Pathology, 2 Urology and 3 Veterinary Anatomy, Justus-Liebig University Giessen, Germany and 4 Department of Histopathology, Imperial College, Hammersmith Campus, London, UK

Abstract

The MUC1 mucin [also known as episialin, epithelial membrane antigen (EMA) or polymorphic epithelial mucin (PEM)] is a component of the mucosal glycocalyx, contributing to anti-adhesive and protective cell functions. MUC1 has been shown in a variety of epithelial cell types in the reproductive tracts of males and females, but this is the first report of its expression in human testis and non-epithelial cells of the germ cell lineage. Analysing 65 testes with normal or impaired spermatogenesis, we identified MUC1 protein in maturing germ cells by immunohistochemistry using the monoclonal antibodies HMFG1, HMFG2 and SM3 binding to different glycosylation variants. MUC1 expression was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis on tissue extracts of human testis, and RT–PCR of selected germ cells after UV laser-assisted cell picking. MUC1 glycosylation variants were selectively distributed during normal spermatogenesis. Whereas HMFG1 labelled certain groups of pachytene spermatocytes, HMFG2 labelled only spermatids. Low glycosylated forms of MUC1 mucin, recognized by SM3, were not found. In contrast to its weak expression during normal spermatogenesis, the HMFG1 glycosylation variant accumulated markedly in all spermatocytes showing abnormal or arrested maturation. These results suggest a variable glycosylation of MUC1 mucin in differentiating germ cells, which is aberrant in pathological conditions.

immunohistochemistry/MUC1/mucins/RT/PCR/spermatogenesis

Notes

5 To whom correspondence should be addressed at: Department of Pathology, Justus-Liebig University of Giessen, Langhansstrasse 10, D-35392 Giessen, Germany. E-mail: Folker.E.Franke{at}patho.med.uni-giessen.de


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