Molecular Human Reproduction, Vol. 7, No. 7, 603-610,
July 2001
© 2001 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Characterization of the genomic organization, localization and expression of four PRY genes (PRY1, PRY2, PRY3 and PRY4)
1 Center for Medical Genetics and 2 Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Laarbeeklaan 101, 1090 Brussels, Belgium
Abstract
PRY (PTP-BL related on the Y chromosome) has been proposed as a candidate spermatogenesis gene. We report the characterization of the genomic structure, the number of copies on the Y chromosome and the expression of the gene. By comparison of the cDNA sequence with the genomic sequence, five exons were identified. Analysis of GenBank-derived clones on the Y chromosome revealed the presence of two full-length copies in azoospermia factor region b (AZFb) (PRY1 and PRY2) and two shorter versions of the PRY gene containing exons 3, 4 and 5 in AZFc (PRY3 and PRY4). A clone containing sequences homologous to exons 3, 4 and 5 is located in area 5L (between AZFa and AZFb), a clone containing a sequence homologous to exon 5 is located in area 5M (in AZFb) and a clone containing a fragment homologous to exon 3 is located in 6F. A repeat structure of exons 1 and 2 is present on the short arm of the Y chromosome as well as on the long arm. PRY1 and PRY2, two gene copies that are located in AZFb, a region often deleted in patients with severe male infertility, were shown to be expressed in the testis. PRY may therefore play an important role in spermatogenesis.
AZF/male infertility/PRY/Y chromosome/Y genes
Notes
3 To whom correspondence should be addressed. E-mail: katrien.stouffs{at}az.vub.ac.be
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Ferras, S. Fernandes, C.J. Marques, F. Carvalho, C. Alves, J. Silva, M. Sousa, and A. Barros AZF and DAZ gene copy-specific deletion analysis in maturation arrest and Sertoli cell-only syndrome Mol. Hum. Reprod., October 1, 2004; 10(10): 755 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tessari, E. Salata, A. Ferlin, L. Bartoloni, M.L. Slongo, and C. Foresta Characterization of HSFY, a novel AZFb gene on the Y chromosome with a possible role in human spermatogenesis Mol. Hum. Reprod., April 1, 2004; 10(4): 253 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Stouffs, W. Lissens, G. Verheyen, L. Van Landuyt, A. Goossens, H. Tournaye, A. Van Steirteghem, and I. Liebaers Expression pattern of the Y-linked PRY gene suggests a function in apoptosis but not in spermatogenesis Mol. Hum. Reprod., January 1, 2004; 10(1): 15 - 21. [Abstract] [Full Text] [PDF]
|
|