Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Thiruppathi, P.
Right arrow Articles by Luborsky, J.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thiruppathi, P.
Right arrow Articles by Luborsky, J.L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 7, No. 8, 697-704, August 2001
© 2001 European Society of Human Reproduction and Embryology

Reproductive endocrinology

Gonadotrophin receptor expression on human granulosa cells of low and normal responders to FSH

P. Thiruppathi1, S. Shatavi1, J.A. Dias2, E. Radwanska1 and J.L. Luborsky1,3

1 Department of Obstetrics and Gynecology, Rush Medical College, Chicago, IL and 2 Wadsworth Center, New York State Department of Health, Albany, NY, USA

Abstract

The aim of this study was to determine if follicle stimulating hormone receptor and luteinizing hormone receptor (FSH-R and LH-R) expression is altered on granulosa cells (GC) of women with low oestradiol responses to FSH. Cells were obtained from mature follicles (>17 mm) following controlled ovarian stimulation. For comparison, chinese hamster ovary (CHO) cells transfected with FSH-R or LH-R were also assessed. FSH-R and LH-R expression were detected by flow cytometry. Receptors were labelled with FSH-R antibodies, or with excess FSH or human chorionic gonadotrophin (HCG) and anti-FSH or HCG antibodies, and compared to multiple controls. Receptor expression on GCs was more heterogeneous than on CHO cells. Gonadotrophin receptor levels on GCs were not correlated with the number of FSH ampoules administered or peak oestradiol response. Low and normal response groups were defined using a ratio of peak oestradiol/number of FSH ampoules. FSH receptor expression was not different on GCs from low and normal responders. However, LH-R expression was higher on GCs of low responders compared to those of normal responders (P = 0.04 ) suggesting a trend to more advanced luteinization. Access of hormone to follicles was not reduced in low responders. Thus, differences in gonadotrophin receptor expression, hormone binding, and access of hormones to follicles do not appear to account for low oestradiol responses to FSH.

FSH/LH/low responders/receptors

Notes

3 To whom correspondence should be addressed at: Rush Medical College, 1653 W Congress Parkway, Chicago, IL 66012, USA.E-mail: jluborsk{at}rush.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
A. Requena, J. Herrero, J. Landeras, E. Navarro, J. L. Neyro, C. Salvador, R. Tur, J. Callejo, M. A. Checa, M. Farre, et al.
Use of letrozole in assisted reproduction: a systematic review and meta-analysis
Hum. Reprod. Update, November 1, 2008; 14(6): 571 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J.L. Luborsky, P. Thiruppathi, B. Rivnay, R. Roussev, C. Coulam, and E. Radwanska
Evidence for different aetiologies of low estradiol response to FSH: age-related accelerated luteinization of follicles or presence of ovarian autoantibodies
Hum. Reprod., October 1, 2002; 17(10): 2641 - 2649.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.