Molecular Human Reproduction, Vol. 7, No. 8, 741-746,
August 2001
© 2001 European Society of Human Reproduction and Embryology
Uterine physiology |
Expression and regulation of growth-regulated oncogene 
in human endometrial stromal cells
Department of Obstetrics and Gynecology, Oita Medical University, Hasama-machi, Oita 879-5593, Japan
Abstract
Growth-regulated oncogene 
(GRO), a potent chemoattractant for neutrophils, has previously been detected in the endometrial stromal cells (ESC) of human endometrium. In this study, the mRNA expression of GRO in the endometrium was evaluated by reverse transcription–polymerase chain reaction analysis, while the localization of GRO protein was studied by immunohistochemistry and the concentrations of GRO were measured using an enzyme-linked immunosorbent assay (ELISA). The effects of known modulators of endometrial function on the production of GRO by ESC were also examined by ELISA and Northern blot analysis. The expression of both GRO mRNA and GRO protein was detected in the cycling endometrium. GRO protein was localized mainly in the stroma, and endometrial tissues in the secretory phase contained higher amounts of GRO protein than did those in the proliferative phase. The production of GRO by ESC was enhanced by in-vitro decidualization. Lipopolysaccharide, tumour necrosis factor- and interleukin-1ß also stimulated the expression of GRO mRNA and protein by ESC. These results suggest that the production of GRO by ESC is regulated by ovarian steroid hormones as well as by inflammatory mediators. The modulation of GRO concentrations in the local environment may contribute to normal and pathological processes in the uterus by regulating leukocyte trafficking in the endometrium.
cytokine network/endometrial stromal cell/growth-regulated oncogene /menstrual cycle
Notes
1 To whom correspondence should be addressed. E-mail: nasu{at}oita-med.ac.jp
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