Molecular Human Reproduction, Vol. 7, No. 8, 787-790,
August 2001
© 2001 European Society of Human Reproduction and Embryology
Implantation and pregnancy |
Nuclear factor-kappa B is essential for up-regulation of interleukin-8 expression in human amnion and cervical epithelial cells
1 Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, Queen Charlotte's Hospital, Goldhawk Road, London W6 0XG, UK and 2 Division of Gastroenterology, Department of Internal Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6144, USA.
Abstract
Interleukin-8 (IL-8) is a cytokine which recruits and activates neutrophils into tissue stroma. It is present in uterine tissues and its concentration increases in the third trimester and with labour. The promoter region of the
IL-8
gene contains binding sites for the transcription factors, nuclear factor-kappa B (NF-
B), activator protein-1 (AP-1) and CCAAT/enhancer-binding protein (C/EBP). These are in close proximity to each other and to the coding region of the gene. This study used site-directed mutagenesis of each of these sites to examine the relative importance of each site in
IL-8
gene expression in a cervical cell line and in amnion cells obtained before and after labour. We found that the NF-B site was essential for basal and IL-1ß-stimulated gene expression in all cell types. Neither of the other binding sites was consistently essential for gene expression but may have an additive role in promoter activity. We conclude that the NF-B binding site is essential for up-regulation of
IL-8
gene expression in these uterine cell types. An increase in IL-8 expression has been shown to occur in the uterus in association with parturition and NF-B binding to the promoter may be of importance at this time.
amnion/cervix/interleukin-8/NF-kB/parturition
Notes
3 To whom correspondence should be addressed. E-mail: c.elliott{at}ic.ac.uk
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. E Ackerman IV, T. L.S Summerfield, D. D Vandre, J. M Robinson, and D. A Kniss Nuclear Factor-Kappa B Regulates Inducible Prostaglandin E Synthase Expression in Human Amnion Mesenchymal Cells Biol Reprod, January 1, 2008; 78(1): 68 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Marx, M. J. Wentz, L. B. MacKay, D. Schlembach, H. Maul, C. Fittkow, R. Given, Y. Vedernikov, G. R. Saade, and R. E. Garfield Effects of Progesterone on iNOS, COX-2, and Collagen Expression in the Cervix J. Histochem. Cytochem., June 1, 2006; 54(6): 623 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Terzidou, Y. Lee, T. Lindstrom, M. Johnson, S. Thornton, and P. R. Bennett Regulation of the Human Oxytocin Receptor by Nuclear Factor-{kappa}B and CCAAT/Enhancer-Binding Protein-{beta} J. Clin. Endocrinol. Metab., June 1, 2006; 91(6): 2317 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Slater, S. Astle, N. Woodcock, J. E. Chivers, N. C.J. de Wit, S. Thornton, M. Vatish, and R. Newton Anti-inflammatory and relaxatory effects of prostaglandin E2 in myometrial smooth muscle Mol. Hum. Reprod., February 1, 2006; 12(2): 89 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M Lindstrom and P. R Bennett The role of nuclear factor kappa B in human labour Reproduction, November 1, 2005; 130(5): 569 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Chapman, I. Smyrnias, D. O. C. Anumba, G. N. Europe-Finner, and S. C. Robson Expression of the GTP-Binding Protein (G{alpha}s) Is Repressed by the Nuclear Factor {kappa}B RelA Subunit in Human Myometrium Endocrinology, November 1, 2005; 146(11): 4994 - 5002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Loor, H. M. Dann, R. E. Everts, R. Oliveira, C. A. Green, N. A. J. Guretzky, S. L. Rodriguez-Zas, H. A. Lewin, and J. K. Drackley Temporal gene expression profiling of liver from periparturient dairy cows reveals complex adaptive mechanisms in hepatic function Physiol Genomics, October 17, 2005; 23(2): 217 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.A.Z. Loudon, S.R. Sooranna, P.R. Bennett, and M.R. Johnson Mechanical stretch of human uterine smooth muscle cells increases IL-8 mRNA expression and peptide synthesis Mol. Hum. Reprod., December 1, 2004; 10(12): 895 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Chapman, G. N. Europe-Finner, and S. C. Robson Expression and Deoxyribonucleic Acid-Binding Activity of the Nuclear Factor {kappa}B Family in the Human Myometrium during Pregnancy and Labor J. Clin. Endocrinol. Metab., November 1, 2004; 89(11): 5683 - 5693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lappas, M. Permezel, H. M. Georgiou, and G. E. Rice Regulation of Phospholipase Isozymes by Nuclear Factor-{kappa}B in Human Gestational Tissues in Vitro J. Clin. Endocrinol. Metab., May 1, 2004; 89(5): 2365 - 2372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Yellon, A. M. Mackler, and M. A. Kirby The Role of Leukocyte Traffic and Activation in Parturition Reproductive Sciences, September 1, 2003; 10(6): 323 - 338. [Abstract] [PDF]
|
|
|
|
|
|
J. A.Z. Loudon, C. L. Elliott, F. Hills, and P. R. Bennett Progesterone Represses Interleukin-8 and Cyclo-Oxygenase-2 in Human Lower Segment Fibroblast Cells and Amnion Epithelial Cells Biol Reprod, July 1, 2003; 69(1): 331 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lappas, M. Permezel, and G. E. Rice N-Acetyl-Cysteine Inhibits Phospholipid Metabolism, Proinflammatory Cytokine Release, Protease Activity, and Nuclear Factor-{kappa}B Deoxyribonucleic Acid-Binding Activity in Human Fetal Membranes in Vitro J. Clin. Endocrinol. Metab., April 1, 2003; 88(4): 1723 - 1729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lee, V. Allport, A. Sykes, T. Lindstrom, D. Slater, and P. Bennett The effects of labour and of interleukin 1 beta upon the expression of nuclear factor kappa B related proteins in human amnion Mol. Hum. Reprod., April 1, 2003; 9(4): 213 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Watari, H. Watari, T. Fujimoto, H. Yamada, J. Nishihira, J. f. Strauss III, and S. Fujimoto Lipopolysaccharide Induces Interleukin-8 Production By Human Cervical Smooth Muscle Cells Reproductive Sciences, February 1, 2003; 10(2): 110 - 117. [Abstract] [PDF]
|
|
|
|
|
|
S. Cauci, S. Guaschino, D. de Aloysio, S. Driussi, D. De Santo, P. Penacchioni, and F. Quadrifoglio Interrelationships of interleukin-8 with interleukin-1{beta} and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women Mol. Hum. Reprod., January 1, 2003; 9(1): 53 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lappas, M. Permezel, H. M. Georgiou, and G. E. Rice Nuclear Factor Kappa B Regulation of Proinflammatory Cytokines in Human Gestational Tissues In Vitro Biol Reprod, August 1, 2002; 67(2): 668 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Romero, H. Kuivaniemi, and G. Tromp Functional Genomics and Proteomics in Term and Preterm Parturition J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2431 - 2434. [Full Text] [PDF]
|
|