Molecular Human Reproduction, Vol. 7, No. 9, 829-837,
September 2001
© 2001 European Society of Human Reproduction and Embryology
Embryology |
The effects of antibodies to heat shock protein 70 in fertilization and embryo development
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada
Abstract
The role of heat shock proteins in shielding organisms from environmental stress is illustrated by the large-scale synthesis of these proteins by the organisms studied to date. However, recent evidence also suggests an important role for heat shock proteins in fertilization and early development of mammalian embryos. We found that the presence of anti-HSP70 antibody significantly reduced tight binding of spermatozoa to the zona pellucida of bovine oocytes and interrupted completion of meiosis II and pronuclear formation. Furthermore, the presence of anti-HSP70 in culture medium from day 3 to day 9 of development increased apoptosis and significantly reduced the number of embryos reaching the blastocyst stage. We further observed that the proportion of apoptotic cells in bovine blastocysts was significantly lower after in-vitro culture with a prior exposure to increased temperature. However, nuclear localization of the p53 protein, which is thought to be essential for the up-regulation of genes involved in apoptosis and cell cycle arrest, was detected in the majority of nuclei in blastocysts exposed to increased temperature, whereas in their untreated (control) counterparts, p53 protein was only detected in the cytoplasm. The decrease in apoptosis after exposure of blastocysts to increased temperature may be attributed to cell cycle arrest resulting from nuclear localization of the p53 protein and/or to an increase in heat shock protein synthesis. We propose that HSP70 plays a critical role in fertilization and early embryonic development.
apoptosis/blastocyst/fertilization/HSP70/p53
Notes
1 To whom correspondence should be addressed. E-mail: waking{at}uoguelph.ca
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. C Wilkerson and K. D Sarge RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa Reproduction, June 1, 2009; 137(6): 923 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.H. Betts and P. Madan Permanent embryo arrest: molecular and cellular concepts Mol. Hum. Reprod., August 1, 2008; 14(8): 445 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Wilkerson, L. A. Murphy, and K. D. Sarge Interaction of HSF1 and HSF2 with the Hspa1b Promoter in Mouse Epididymal Spermatozoa Biol Reprod, August 1, 2008; 79(2): 283 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.A. Mitchell, B. Nixon, and R.J. Aitken Analysis of chaperone proteins associated with human spermatozoa during capacitation Mol. Hum. Reprod., September 1, 2007; 13(9): 605 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Spinaci, S. Volpe, C. Bernardini, M. de Ambrogi, C. Tamanini, E. Seren, and G. Galeati Sperm Sorting Procedure Induces a Redistribution of Hsp70 but Not Hsp60 and Hsp90 in Boar Spermatozoa J Androl, November 1, 2006; 27(6): 899 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rodriguez, C. Diez, S. Ikeda, L.J. Royo, J.N. Caamano, C. Alonso-Montes, F. Goyache, I. Alvarez, N. Facal, and E. Gomez Retinoids during the in vitro transition from bovine morula to blastocyst Hum. Reprod., August 1, 2006; 21(8): 2149 - 2157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.S. Xu, Y.L. Lee, K.F. Lee, K.L. Kwok, W.M. Lee, J.M. Luk, and W.S.B. Yeung Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the {beta}1 subunit of sodium-potassium ATPase in mouse embryos Hum. Reprod., December 1, 2004; 19(12): 2919 - 2926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. O. Gjorret, H. M. Knijn, S. J. Dieleman, B. Avery, L.-I. Larsson, and P. Maddox-Hyttel Chronology of Apoptosis in Bovine Embryos Produced In Vivo and In Vitro Biol Reprod, October 1, 2003; 69(4): 1193 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.L. Lee, K.F. Lee, J.S. Xu, K.L. Kwok, J.M. Luk, W.M. Lee, and W.S.B. Yeung Embryotrophic Factor-3 from Human Oviductal Cells Affects the Messenger RNA Expression of Mouse Blastocyst Biol Reprod, February 1, 2003; 68(2): 375 - 382. [Abstract] [Full Text] [PDF]
|
|