Molecular Human Reproduction, Vol. 7, No. 9, 887-894,
September 2001
© 2001 European Society of Human Reproduction and Embryology
Implantation and pregnancy |
Transcriptional regulation of human placental leucine aminopeptidase/oxytocinase gene
1 Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya, 466-8550, Japan and 2 Laboratory of Cellular Biochemistry, Institute of Physical and Chemical Research (RIKEN), Saitama, 351-0148, Japan
Abstract
Human placental leucine aminopeptidase (P-LAP) plays a major role in the clearance of oxytocin, which is a key hormone in regulating labour pain. To explore the transcriptional regulation of P-LAP gene expression in placenta, we performed systematic studies using human choriocarcinoma cells, BeWo and JEG-3, as a model of placental trophoblastic cells. Transient transfection and luciferase assays using various 5'-deleted P-LAP-luciferase constructs showed that the region from –297 to +49 of the transcription start site was responsible for promoter activity in these cells. Footprinting analysis with nuclear extracts from both cell lines demonstrated at least four sites for nucleoprotein interactions in this region (FP1 to FP4). Site-directed deletion of FP1–4 in luciferase assays indicated the significance of the FP3 region (–214 to –183) for high promoter activity in the cells. Electrophoretic mobility shift assays to identify the proteins interacting with DNA at FP3 revealed three retarded bands, one of which was generated by activator protein-2 (AP-2) binding. Our findings suggest that AP-2 may be one of the important factors regulating P-LAP gene expression in human placenta.
aminopeptidase/AP-2/oxytocinase/placenta/promoter
Notes
3 To whom correspondence should be addressed. E-mail: snomura{at}med.nagoya-u.ac.jp
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