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Molecular Human Reproduction, Vol. 8, No. 1, 16-23, January 2002
© 2002 European Society of Human Reproduction and Embryology

Testis and spermatogenesis

Cloning and characterization of a human orthologue of testis-specific succinyl CoA: 3-oxo acid CoA transferase (Scot-t) cDNA

Hiromitsu Tanaka, Junya Kohroki, Naoko Iguchi, Masayoshi Onishi and Yoshitake Nishimune,1

Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University,3–1 Yamadaoka, Suita City, Osaka 565-0871, Japan

Succinyl CoA: 3-oxo acid CoA transferase (scot; EC 2.8.3.5) is a key enzyme for metabolism of ketone bodies. Previously, we have cloned a testis-specific succinyl CoA: 3-oxo acid CoA transferase (scot-t) cDNA from a subtracted cDNA library of mouse testis and demonstrated its expression to be specific to late haploid male germ cells. In this study, the human orthologue of mouse scot-t was cloned and characterized. The entire coding region of the mRNA and the deduced amino acid sequence of human Scot-t (h-Scot-t) showed 75.4 and 75.8% identity with mouse scot-t respectively. The mRNA was exclusively expressed in the testis, and the protein was localized to the midpiece of ejaculated spermatozoa where mitochondria exist. We showed that the h-Scot-t gene was intronless by using a polymerase chain reaction technique and that a non-functional pseudogene, 98% similar to h-Scot-t, was also located on the human genome (1p33-34.3). Furthermore, the genomic structure of the actual h-Scot-t transcription unit was found to be located in an intron (1p34.1–35.3) of the bone morphogenetic protein 8 (BMP8) gene. In conclusion, we have demonstrated that h-Scot-t is a single intronless gene specifically expressed in the testis.

intronless/OXCT/Scot/spermatozoa/testis

1 To whom correspondence should be addressed. E-mail: nishimun{at}biken.osaka-u.ac.jp


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