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Molecular Human Reproduction, Vol. 8, No. 1, 24-31, January 2002
© 2002 European Society of Human Reproduction and Embryology

Testis and spermatogenesis

Molecular characterization of the TCP11 gene which is the human homologue of the mouse gene encoding the receptor of fertilization promoting peptide

Yongxin Ma1,2, Sizhong Zhang1,2,5, Qinjie Xia1,2, Ge Zhang1,2, Xinli Huang4, Mingkong Huang3, Cuiying Xiao1,2, Agen Pan4, Yan Sun1,2, Roger Lebo4 and Aubrey Milunsky3

1 Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, 610041, 2 Key Laboratory of Morbid Genomics and Forensic Medicine, Sichuan Province, 3 Department of Urology, Sichuan College of Genital Health, Sichuan Province, China and 4 Center for Human Genetics, Boston University, Boston, MA 02118, USA

A human testis-specific gene was isolated by subtractive hybridization between the cDNA pools of adult and fetal testes, followed by rapid amplification of cDNA ends (RACE). This gene sequence is highly homologous to a large portion of the mouse Tcp11 gene which is important in sperm function because it encodes the receptor for fertilization-promoting peptide (FPP). The gene was mapped to human chromosome band 6p21 by fluorescence in-situ hybridization. The 9 exon gene spans a 22.8 kp genomic DNA sequence. The mature processed message encodes a 441 amino acid protein that is highly homologous to the mouse 566 amino acid protein after the first 142 amino acids. Results of Northern blot and RT–PCR analyses of RNA extracted from human tissues revealed that the gene is only expressed in fertile adult testes, but not in azoospermic testes, fetal testes nor in other human tissues. Taken together, our results along with the mouse Tcp11 function suggest that TCP11 gene is important in sperm function and fertility.

fertilization promoting peptide/human TCP11/mouse Tcp11/testis gene expression

5 To whom correspondence should be addressed. E-mail: szzhang{at}mcwcums.com


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