Molecular Human Reproduction, Vol. 8, No. 10, 893-899,
October 2002
© 2002 European Society of Human Reproduction and Embryology
Reproductive endocrinology |
Genetic and functional analyses of polymorphisms in the human FSH receptor gene
1 Department of Obstetrics and Gynecology, Hokkaido University, School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan and 2 Department of Gynecology and Obstetrics, Division of Reproductive Biology, Stanford University Medical Center, Stanford, CA 94305-5317, USA
To determine the influence of FSH receptor variants Thr307-Asn680 (TN) and Ala307-Ser680 (AS) on ovarian function, we investigated the frequency of these gene polymorphisms by using restriction fragment length polymorphism analysis and observed their effects on clinical manifestations. In a population of 522 Japanese women, the overall frequency of TN/TN (NN), TN/AS (NS), and AS/AS (SS) was 41.0, 46.9 and 12.1% respectively. In polycystic ovary patients, the NS population was significantly larger when compared with the spontaneously ovulating group (66.7 versus 43.5%, P < 0.05). In the SS group, a significantly higher (46%) basal level of serum FSH was observed as compared with that in the NS group (P < 0.05). A higher dose of the exogenous gonadotrophin was required to achieve ovulation induction in the SS group as compared with the NS group (P < 0.05). At the time of hCG administration, estradiol levels per oocyte retrieved for IVF in the SS group were significantly lower as compared with the levels in the NS and NN groups (P < 0.05). There were no significant differences in FSH-stimulated cAMP production and PI turnover as well as ligand-binding affinity between the two receptor isoforms when overexpressed in transfected 293T cells. These results suggest that although FSH receptor polymorphisms have no discernible effect on FSH receptor function in vitro, there are associations between the genotype and some aspects of patient status.
FSH receptor/ovarian function/polycystic ovary/polymorphism/RFLP
3 To whom correspondence should be addressed. E-mail: mkudo{at}med.hokudai.ac.jp
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