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Molecular Human Reproduction, Vol. 8, No. 10, 906-911, October 2002
© 2002 European Society of Human Reproduction and Embryology

Ovary and oogenesis

Growth factors promote meiosis in mouse fetal ovaries in vitro

S. Lyrakou, M.A. Hultén and G.M. Hartshorne1

Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

Mouse fetal ovaries were cultured to investigate germ cell development in the presence of a combination of the growth factors (GFs) stem cell factor, insulin-like growth factor-1 and leukaemia inhibitory factor. Ovaries were isolated from fetal mice at 13 and 14 days post-coitum (dpc) and cultured to the equivalent of 17 dpc. Culture conditions comprised minimal essential medium-{alpha} plus 5% fetal calf serum, with or without GFs. Oocytes were assessed using immunofluorescence to illustrate synaptonemal complexes and recombination foci. The proportions of pachytene cells in freshly isolated 13, 14 and 17 dpc ovaries were 0, 8 and 74% respectively. There was a significant (P < 0.0001) increase in the number of pachytene cells after 4 days culture with GFs, with 24% of germ cells from 13 dpc ovaries reaching pachytene. In contrast, no pachytene cells were detected in cultures of 13 dpc ovaries without GFs. After 3 days in culture with GFs, 38% of germ cells from 14 dpc ovaries were at pachytene compared with 19% without GFs. In conclusion, we have demonstrated positive effects of GFs upon oocyte formation by meiosis in vitro. The observed results could be explained by an increased survival of premeiotic oogonia entering meiosis, or by effects on oocytes already in early meiosis.

growth factors/meiosis/MLH1/mouse/oocyte

1 To whom correspondence should be addressed at: 23 Butterworth Drive, Coventry, CV4 8JL (until 1.11.02), and the address above thereafter. E-mail : ghartshorne{at}bio.warwick.ac.uk


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