Molecular Human Reproduction, Vol. 8, No. 10, 923-929,
October 2002
© 2002 European Society of Human Reproduction and Embryology
Embryology |
Sex-chromosome linked gene expression in in-vitro produced bovine embryos
1 MTT, Agrifood Research Finland, Animal Production Research, Animal Breeding, FIN-31600 Jokioinen, Finland, 2 Department of Biomedical Sciences, University of Guelph, Guelph, ON, N1G 2W1 Canada and 3 Finnish Game and Fisheries Research Institute, PO Box 6, FIN-00721 Helsinki, Finland
The expression of XIST, G6PD, HPRT, ZFX and ZFY were investigated in in-vitro produced bovine embryos. Transcripts of these genes were assayed by RT–PCR in pools of pre-compaction stage embryos and sexed pools of morulae and blastocysts. The expression of XIST, G6PD, HPRT and ZFX in female and male morulae and blastocysts were compared using a semi-quantitative RT–PCR. G6PD, HPRT and ZFX transcripts were noted in all pre-compaction stage embryos and in female and male blastocysts. ZFY transcripts were detected in unsexed pools of 8–16-cell stage embryos and in male blastocysts. XIST transcripts were detected in unsexed pools at the 8–16 cell stage, in male and female morulae, and in female blastocysts. The level of XIST RNA was significantly higher in female morulae than in males. Levels of G6PD and HPRT RNA were also higher in female morulae and blastocysts than in males, but only G6PD levels were significantly different between the sexes. The expression of ZFX was also significantly higher in female than in male blastocysts. These results show sexually dimorphic expression of sex chromosome linked genes prior to the blastocyst stage in in-vitro produced bovine embryos.
G6PD/HPRT/sex-related growth rate/XIST/ZFX/Y
4 Present address: Leiras OY, Research & Development, Clinical Research, PO Box 415, FIN-20101 Turku, Finland
5 To whom correspondence should be addressed. E-mail: jaana.peippo{at}mtt.fi
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