Molecular Human Reproduction, Vol. 8, No. 10, 946-951,
October 2002
© 2002 European Society of Human Reproduction and Embryology
Implantation and pregnancy |
Molecular identification and localization of Trp homologues, putative calcium channels, in pregnant human uterus
1 Parturition Research Group, Maternal and Fetal Research Unit, Department of Women’s Health, 10th Floor North Wing, Guy’s, King’s and St Thomas’ School of Medicine, St Thomas’ Hospital Campus, Lambeth Palace Road, London SE1 7EH, 2 Department of Biological Sciences, The University of Warwick, Coventry CV4 7AL and 3 School of Biomedical Sciences, Worsley Building, Level 9, University of Leeds, Leeds LS2 9JT, UK
The mechanisms underlying the switch from uterine quiescence to contractile activity in labour are not clearly understood. Increasing evidence suggests that pathways of myometrial calcium homeostasis, including store-operated calcium entry (SOCE), may play an important role. The molecular basis of the membrane-associated calcium channels contributing to SOCE in pregnant human myometrium is not known, but they are likely to be hetero- or homo-oligomeric assemblies of transient receptor potential channel (TrpC) proteins, encoded by the mammalian homologues of Drosophila Trp genes. This study has therefore determined Trp gene expression and also TrpC protein expression and localization in term pregnant human myometrial tissue and primary cultured human myometrial smooth muscle (HMSM) cells. RT–PCR amplified fragments of Trp1, Trp3, Trp4, Trp6 and Trp7. PCR products were 100% homologous to published human sequences. Western blot analysis detected TrpC1, TrpC3, TrpC4 and TrpC6 proteins, which were of expected size. Immunolocalization revealed TrpC1, TrpC3, TrpC4 and TrpC6 protein expression in myometrial tissue and HMSM cells. TrpC protein immunostaining in HMSM cells was distributed in a distinct reticular fashion. TrpC proteins may be candidate proteins forming SOCE channels in term pregnant human myometrium.
calcium/myometrium/smooth muscle/SOCE/TrpC
4 To whom correspondence should be addressed. E-mail: rachel.tribe{at}kcl.ac.uk
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Sekaran, G. S. Lall, K. L. Ralphs, A. J. Wolstenholme, R. J. Lucas, R. G. Foster, and M. W. Hankins 2-Aminoethoxydiphenylborane Is an Acute Inhibitor of Directly Photosensitive Retinal Ganglion Cell Activity In Vitro and In Vivo J. Neurosci., April 11, 2007; 27(15): 3981 - 3986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dalrymple, K. Mahn, L. Poston, E. Songu-Mize, and R.M. Tribe Mechanical stretch regulates TRPC expression and calcium entry in human myometrial smooth muscle cells Mol. Hum. Reprod., March 1, 2007; 13(3): 171 - 179*. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Morales, A. Diez, A. Puyet, P. J. Camello, C. Camello-Almaraz, J. M. Bautista, and M. J. Pozo Calcium controls smooth muscle TRPC gene transcription via the CaMK/calcineurin-dependent pathways Am J Physiol Cell Physiol, January 1, 2007; 292(1): C553 - C563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Y. Ku, L. Babich, R. A. Word, M. Zhong, A. Ulloa, M. Monga, and B. M. Sanborn Expression of Transient Receptor Channel Proteins in Human Fundal Myometrium in Pregnancy Reproductive Sciences, April 1, 2006; 13(3): 217 - 225. [Abstract] [PDF]
|
|
|
|
|
|
D. M. Slater, S. Astle, N. Woodcock, J. E. Chivers, N. C.J. de Wit, S. Thornton, M. Vatish, and R. Newton Anti-inflammatory and relaxatory effects of prostaglandin E2 in myometrial smooth muscle Mol. Hum. Reprod., February 1, 2006; 12(2): 89 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ay, A. Iyanoye, G. C. Sieck, Y. S. Prakash, and C. M. Pabelick Cyclic nucleotide regulation of store-operated Ca2+ influx in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L278 - L283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Sanborn, C.-Y. Ku, S. Shlykov, and L. Babich Molecular Signaling Through G-Protein-Coupled Receptors and the Control of Intracellular Calcium in Myometrium Reproductive Sciences, October 1, 2005; 12(7): 479 - 487. [Abstract] [PDF]
|
|
|
|
 |
|
 V. A. Golovina Visualization of localized store-operated calcium entry in mouse astrocytes. Close proximity to the endoplasmic reticulum J. Physiol., May 1, 2005; 564(3): 737 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bailey, A. J Tyson-Capper, K. Gilmore, S. C Robson, and G N. Europe-Finner Identification of human myometrial target genes of the cAMP pathway: the role of cAMP-response element binding (CREB) and modulator (CREM{alpha} and CREM{tau}2{alpha}) proteins J. Mol. Endocrinol., February 1, 2005; 34(1): 1 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bailey and G N. Europe-Finner Identification of human myometrial target genes of the c-Jun NH2-terminal kinase (JNK) pathway: the role of activating transcription factor 2 (ATF2) and a novel spliced isoform ATF2-small J. Mol. Endocrinol., February 1, 2005; 34(1): 19 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhang, C. V. Remillard, I. Fantozzi, and J. X.-J. Yuan ATP-induced mitogenesis is mediated by cyclic AMP response element-binding protein-enhanced TRPC4 expression and activity in human pulmonary artery smooth muscle cells Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1192 - C1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Beech, K. Muraki, and R. Flemming Non-selective cationic channels of smooth muscle and the mammalian homologues of Drosophila TRP J. Physiol., September 15, 2004; 559(3): 685 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ay, Y. S. Prakash, C. M. Pabelick, and G. C. Sieck Store-operated Ca2+ entry in porcine airway smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L909 - L917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, L. A. Shimoda, and J. T. Sylvester Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L848 - L858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Babich, C.-Y. Ku, H. W.J. Young, H. Huang, M. R. Blackburn, and B. M. Sanborn Expression of Capacitative Calcium TrpC Proteins in Rat MyometriumDuring Pregnancy Biol Reprod, April 1, 2004; 70(4): 919 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dalrymple, D. M. Slater, L. Poston, and R. M. Tribe Physiological Induction of Transient Receptor Potential Canonical Proteins, Calcium Entry Channels, in Human Myometrium: Influence of Pregnancy, Labor, and Interleukin-1{beta} J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1291 - 1300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Shlykov, M. Yang, J. L. Alcorn, and B. M. Sanborn Capacitative Cation Entry in Human Myometrial Cells and Augmentation by hTrpC3 Overexpression Biol Reprod, August 1, 2003; 69(2): 647 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wrayzx, K. Jones, S. Kupittayanant, Y. Li, A. Matthew, E. Monir-Bishty, K. Noble, S. J. Pierce, S. Quenby, and A. V. Shmygol Calcium Signaling and Uterine Contractility Reproductive Sciences, July 1, 2003; 10(5): 252 - 264. [Abstract] [PDF]
|
|