Molecular Human Reproduction, Vol. 8, No. 3, 237-245,
March 2002
© 2002 European Society of Human Reproduction and Embryology
Embryology |
Expression of cell adhesion molecules during human preimplantation embryo development
1 School of Biological Sciences, University of Manchester, Manchester M13 9PT, 2 Department of Reproductive Medicine,Leeds General Infirmary, Leeds, LS2 9NS, 3 Department of Reproductive Medicine, St Mary's Hospital, Manchester, M13 0JH, UK
Abstract
Formation of a fully differentiated, implantation competent blastocyst requires the expression of a complex repertoire of molecules. However, the events that drive morphogenesis are poorly elucidated in the human embryo. In this work, we describe the amplification of representative cDNAs from morphologically and developmentally normal, individual human embryos at all stages from pronucleate to blastocyst. These cDNAs were probed to reveal the temporal expression pattern of cell adhesion molecules thought to play a key role in murine preimplantation embryo development. We demonstrated constitutive expression of ß actin, ß1 and 
6 integrins, ZO-1 and E-cadherin, as shown previously in mouse embryos. No expression of ß3, 2, 3 or 7 integrins nor of L or P selectin was detected at any stage of preimplantation development. ß5 integrin showed a regulated pattern of expression and was not expressed in blastocysts, while desmocollin-2 could only be detected at the blastocyst stage. Expression and localization of ß1, ß5 and 6 integrins and ZO-1 and E-cadherin proteins was confirmed in blastocyst stage embryos by immunocytochemistry. We have identified differences in the expression of integrin molecules between mouse and human embryos, and propose a role for vß5 and 6ß1 integrin dimers in the human embryo at implantation.
cell adhesion/embryo/human/integrin/preimplantation
Notes
4 To whom correspondence should be addressed. E-mail: sue.kimber{at}man.ac.uk
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