Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wu, X.
Right arrow Articles by Flores-Morales, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, X.
Right arrow Articles by Flores-Morales, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 8, No. 3, 246-254, March 2002
© 2002 European Society of Human Reproduction and Embryology

Uterine physiology

Identification of genes with higher expression in human uterine leiomyomas than in the corresponding myometrium

Xuxia Wu1,2,3, Agneta Blanck2, Gunnar Norstedt3, Lena Sahlin1 and Amilcar Flores-Morales3,4

1 Division for Reproductive Endocrinology, Department of Woman and Child Health, Karolinska Hospital L5:01, S-171 76 Stockholm, 2 Department of Clinical Sciences, Division for Obstetrics and Gynecology, Huddinge Hospital, S-141 86 Huddinge and 3 Department of Molecular Medicine, Karolinska Hospital L8:01, S 171 76 Stockholm, Sweden

We used a PCR-based subtraction method, representational difference analysis of cDNA (cDNA–RDA), to identify genes with a higher expression in leiomyomas in comparison with the corresponding myometrium during the proliferative phase of the menstrual cycle. Increased expression of the genes for pregnancy-associated plasma protein A (PAPPA), tomoregulin, cellular retinoid acid binding protein 1 (CRABP1), zinc finger protein 185 (ZFP 185) and latent transforming growth factor ß binding protein 2 (LTBP2) was demonstrated in individual leiomyoma samples compared with corresponding myometrium. Additionally, a specific positive immunostaining of LTBP2 was found in the smooth muscle cells of both leiomyomas and myometrium. These genes may be part of previously unidentified molecular mechanisms responsible for the selective growth advantage of leiomyomas compared with myometrium. This work expands our knowledge about the molecular nature of leiomyomas and provides novel candidate genes to further explore in relation to their function during leiomyoma growth.

differential gene expression/leiomyomas/uterus

4 To whom correspondence should be addressed. E-mail: Amilcar.Flores{at}molmed.ki.se


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
Y. Zhao, Y. Wen, M. L. Polan, J. Qiao, and B. H. Chen
Increased expression of latent TGF-{beta} binding protein-1 and fibrillin-1 in human uterine leiomyomata
Mol. Hum. Reprod., May 1, 2007; 13(5): 343 - 349.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
C.D. Swartz, C.A. Afshari, L. Yu, K.E. Hall, and D. Dixon
Estrogen-induced changes in IGF-I, Myb family and MAP kinase pathway genes in human uterine leiomyoma and normal uterine smooth muscle cell lines
Mol. Hum. Reprod., June 1, 2005; 11(6): 441 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. A. Arslan, L. I. Gold, K. Mittal, T.-C. Suen, I. Belitskaya-Levy, M.-S. Tang, and P. Toniolo
Gene expression studies provide clues to the pathogenesis of uterine leiomyoma: new evidence and a systematic review
Hum. Reprod., April 1, 2005; 20(4): 852 - 863.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
G. Weston, A. C. Trajstman, C. E. Gargett, U. Manuelpillai, B. J. Vollenhoven, and P. A.W. Rogers
Fibroids display an anti-angiogenic gene expression profile when compared with adjacent myometrium
Mol. Hum. Reprod., September 1, 2003; 9(9): 541 - 549.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.