Molecular Human Reproduction, Vol. 8, No. 3, 255-261,
March 2002
© 2002 European Society of Human Reproduction and Embryology
Implantation and pregnancy |
Th1- and Th2-like cytokine production by first trimester decidual large granular lymphocytes is influenced by HLA-G and HLA-E
1 Department of Obstetrics and Gynaecology, Julius-Maximilians-University, Wuerzburg and 2 Institute of Anthropology and Human Genetics, Ludwig-Maximilians-University, Munich, Germany
During normal early pregnancy, a particular immune environment in the decidua and the expression of non-classical HLA-G and HLA-E molecules on the invading trophoblast are assumed to be essential for the tolerance of the fetus. To assess whether HLA-G and HLA-E influence the cytokine production of their putative target cells [large granular lymphocytes (LGL)], we analysed the concentrations of tumour necrosis factor (TNF-
), interferon (IFN)-
, interleukin (IL)-10, IL-13 and granulocyte-macrophage colony stimulating factor (GM-CSF) in supernatants of isolated first trimester LGL co-cultured with HLA-G or HLA-E transfected K-562 leukaemia cells lacking the classical HLA class I and II molecules. In comparison with that observed with untransfected K-562 cells, co-culture of LGL with HLA-G-expressing cells significantly reduced the concentration of all cytokines investigated (TNF-, IL-10 and GM-CSF, P < 0.01; IFN- and IL-13, P < 0.05). In contrast, co-culture of LGL with HLA-E-expressing cells significantly (P < 0.01) decreased only IL-10 production, although a strong tendency towards reduced IL-13 levels was also observed. In the co-culture system presented, membrane-bound HLA-G and, to a lesser extent, HLA-E expression affected cytokine release by decidual LGL in a manner not consistent with the Th1/Th2 paradigm. In conclusion, our data are indicative of a general immune-suppressive effect of HLA-G on LGL activity.
cytokine/HLA-E/HLA-G/K-562/LGL
3 To whom correspondence should be addressed at: Universitäts-Frauenklinik, Josef-Schneider-Str. 4, D-97080 Wuerzburg, Germany. E-mail: frak057{at}mail.uni-wuerzburg.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. V. F. Hviid HLA-G in human reproduction: aspects of genetics, function and pregnancy complications Hum. Reprod. Update, May 1, 2006; 12(3): 209 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ntrivalas, J. Kwak-Kim, K. Beaman, H. Mantouvalos, and A. Gilman-Sachs An In Vitro Coculture Model to Study Cytokine Profiles of Natural Killer Cells During Maternal Immune Cell-Trophoblast Interactions Reproductive Sciences, April 1, 2006; 13(3): 196 - 202. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Blaschitz, H. Juch, A. Volz, H. Hutter, C. Daxboeck, G. Desoye, and G. Dohr The soluble pool of HLA-G produced by human trophoblasts does not include detectable levels of the intron 4-containing HLA-G5 and HLA-G6 isoforms Mol. Hum. Reprod., October 1, 2005; 11(10): 699 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.G.M. Lukassen, I. Joosten, B. van Cranenbroek, M.J.C. van Lierop, J. Bulten, D.D.M. Braat, and A. van der Meer Hormonal stimulation for IVF treatment positively affects the CD56bright/CD56dim NK cell ratio of the endometrium during the window of implantation Mol. Hum. Reprod., July 1, 2004; 10(7): 513 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. van der Meer, H.G.M. Lukassen, M.J.C. van Lierop, F. Wijnands, S. Mosselman, D.D.M. Braat, and I. Joosten Membrane-bound HLA-G activates proliferation and interferon-{gamma} production by uterine natural killer cells Mol. Hum. Reprod., March 1, 2004; 10(3): 189 - 195. [Abstract] [Full Text] [PDF]
|
|