Molecular Human Reproduction, Vol. 8, No. 4, 399-408,
April 2002
© 2002 European Society of Human Reproduction and Embryology
Implantation and pregnancy |
Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm
Liggins Institute and Division of Pharmacology & Clinical Pharmacology, Faculty of Medical and Health Sciences, University of Auckland, 85 Park Road, Grafton, Auckland, New Zealand
Inflammatory processes are implicated in preterm labour (PTL). To identify potential novel markers for PTL, we have used commercial cDNA arrays to generate profiles of differential expression of inflammation-associated genes in gestational membranes with term and PTL. RNA for cDNA probe synthesis was isolated from reflected human amnion and choriodecidua membranes delivered following Caesarean section at term before the onset of labour (TNL, n = 4), spontaneous labour at term (TSL, n = 4), and PTL with and without chorioamnionitis (PTL+INF and PTL-INF respectively, n = 4 each). Profiles were displayed relative to TNL and statistical comparisons of TSL versus TNL and PTL+INF versus PTL-INF were performed. Elevated expression of chemokines macrophage inflammatory protein 1ß(MIP–1ß) and pulmonary and activation-regulated chemokine (PARC) was observed in PTL+INF compared to PTL-INF amnion and choriodecidua respectively (P = 0.03). Likewise, the cytokines oncostatin-M and pre-B cell enhancing factor (PBEF) were more highly expressed in PTL+INF compared with PTL-INF and in TSL compared with TNL respectively (P = 0.03). Conversely, inhibin A, tissue inhibitors of matrix metalloproteinase (TIMP)-3 and TIMP-4 were all significantly elevated in PTL-INF compared with PTL+INF (P = 0.03). Furthermore, differential expression patterns of classes of genes, grouped according to function (e.g. chemokines), were noted. The cDNA array approach holds promise for identification of new candidate markers or combinations thereof for prediction or diagnosis of PTL, as well as for increasing our understanding of the particular aetiologies involved.
amnion/chorioamnionitis/cytokines/labour/preterm labour
1 To whom correspondence should be addressed at: Liggins Institute, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. E-mail: k.marvin{at}auckland.ac.nz
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. E. Kendal-Wright Stretching, Mechanotransduction, and Proinflammatory Cytokines in the Fetal Membranes Reproductive Sciences, December 1, 2007; 14(8_suppl): 35 - 41. [Abstract] [PDF]
|
|
|
|
 |
|
 I. Garcia-Verdugo, D. Leiber, P. Robin, E. Billon-Denis, R. Chaby, and Z. Tanfin Direct Interaction of Surfactant Protein A with Myometrial Binding Sites: Signaling and Modulation by Bacterial Lipopolysaccharide Biol Reprod, April 1, 2007; 76(4): 681 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Simon, R. B. Easley, D. N. Grigoryev, S.-F. Ma, S. Q. Ye, T. Lavoie, R. M. Tuder, and J. G. N. Garcia Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L851 - L861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Breuiller-Fouche and G. Germain Gene and protein expression in the myometrium in pregnancy and labor. Reproduction, May 1, 2006; 131(5): 837 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kumar, W. Fung, R. M. Moore, V. Pandey, J. Fox, B. Stetzer, J. M. Mansour, B. M. Mercer, R. W. Redline, and J. J. Moore Proinflammatory Cytokines Found in Amniotic Fluid Induce Collagen Remodeling, Apoptosis, and Biophysical Weakening of Cultured Human Fetal Membranes Biol Reprod, January 1, 2006; 74(1): 29 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M Lindstrom and P. R Bennett The role of nuclear factor kappa B in human labour Reproduction, November 1, 2005; 130(5): 569 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Schutyser, A. Richmond, and J. Van Damme Involvement of CC chemokine ligand 18 (CCL18) in normal and pathological processes J. Leukoc. Biol., July 1, 2005; 78(1): 14 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shankar, N. Gude, F. Cullinane, S. Brennecke, A. W Purcell, and E. K Moses An emerging role for comprehensive proteome analysis in human pregnancy research Reproduction, June 1, 2005; 129(6): 685 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hirsch and H. Wang The Molecular Pathophysiology of Bacterially Induced Preterm Labor: Insights From the Murine Model Reproductive Sciences, April 1, 2005; 12(3): 145 - 155. [Abstract] [PDF]
|
|
|
|
|
|
M. El Khwad, B. Stetzer, R.M. Moore, D. Kumar, B. Mercer, S. Arikat, R.W. Redline, J.M. Mansour, and J.J. Moore Term Human Fetal Membranes Have a Weak Zone Overlying the Lower Uterine Pole and Cervix Before Onset of Labor Biol Reprod, March 1, 2005; 72(3): 720 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Q. Ye, B. A. Simon, J. P. Maloney, A. Zambelli-Weiner, L. Gao, A. Grant, R. B. Easley, B. J. McVerry, R. M. Tuder, T. Standiford, et al. Pre-B-Cell Colony-enhancing Factor as a Potential Novel Biomarker in Acute Lung Injury Am. J. Respir. Crit. Care Med., February 15, 2005; 171(4): 361 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Gayle, R. Beloosesky, M. Desai, F. Amidi, S. E. Nunez, and M. G. Ross Maternal LPS induces cytokines in the amniotic fluid and corticotropin releasing hormone in the fetal rat brain Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2004; 286(6): R1024 - R1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.A. Keelan, J. Yang, R.J. Romero, T. Chaiworapongsa, K.W. Marvin, T.A. Sato, and M.D. Mitchell Epithelial Cell-Derived Neutrophil-Activating Peptide-78 Is Present in Fetal Membranes and Amniotic Fluid at Increased Concentrations with Intra-amniotic Infection and Preterm Delivery Biol Reprod, January 1, 2004; 70(1): 253 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Keelan, K. Wang, T. Chaiworapongsa, R. Romero, M. D. Mitchell, T. A. Sato, D. A. Brown, W. D. Fairlie, and S. N. Breit Macrophage inhibitory cytokine 1 in fetal membranes and amniotic fluid from pregnancies with and without preterm labour and premature rupture of membranes Mol. Hum. Reprod., September 1, 2003; 9(9): 535 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lonergan, D. Aponso, K. W. Marvin, R. J. A. Helliwell, T. A. Sato, M. D. Mitchell, T. Chaiwaropongsa, R. Romero, and J. A. Keelan Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL), TRAIL Receptors, and the Soluble Receptor Osteoprotegerin in Human Gestational Membranes and Amniotic Fluid during Pregnancy and Labor at Term and Preterm J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3835 - 3844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Charpigny, M.-J. Leroy, M. Breuiller-Fouche, Z. Tanfin, S. Mhaouty-Kodja, Ph. Robin, D. Leiber, J. Cohen-Tannoudji, D. Cabrol, C. Barberis, et al. A Functional Genomic Study to Identify Differential Gene Expression in the Preterm and Term Human Myometrium Biol Reprod, June 1, 2003; 68(6): 2289 - 2296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Romero, H. Kuivaniemi, and G. Tromp Functional Genomics and Proteomics in Term and Preterm Parturition J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2431 - 2434. [Full Text] [PDF]
|
|