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Molecular Human Reproduction, Vol. 8, No. 5, 485-493, May 2002
© 2002 European Society of Human Reproduction and Embryology

Implantation and pregnancy

Human choriocarcinoma cell line JEG-3 produces and secretes active retinoids from retinol

Loïc Blanchon1, Patrick Sauvant2, Claes Bavik5, Denis Gallot4, Françoise Charbonne3, Marie-Cecile Alexandre-Gouabau2, Didier Lemery4, Bernard Jacquetin4, Bernard Dastugue1, Simon Ward5 and Vincent Sapin1,6

1 INSERM U.384, Faculté de Médecine, BP 38, 63000 Clermont-Ferrand, 2 INRA, Unité des Maladies Métaboliques et Micronutriments, Equipe Vitamines, Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, 3 Laboratoire de Virologie Médicale, CHU, 4 Médecine Materno-Foetale, Maternité de l'Hôtel-Dieu, 63000 Clermont-Ferrand, France and 5 Biomedical Genetics, University of Sheffield Medical School, Royal Hallamshire Hospital Sheffield, South Yorks, S10 2RX, UK

Vitamin A (retinol) and its active derivatives (the retinoids) are essential for growth and development of the mammalian fetus. Maternally-derived retinol has to pass through the placenta to reach the developing fetus. Despite its apparent importance, little is known about placental metabolism of retinol, and particularly placental production and/or secretion of active retinoids. It has been previously considered that retinoids are recruited from the uterine environment to influence placental development and function during gestation. We have studied retinoid metabolism in the human choriocarcinoma cell line JEG-3 and demonstrate, for the first time, that active retinoids are produced endogenously by the JEG-3 cell line from retinol. These retinoids induce gene expression from a retinoic acid-responsive enhancer element reporter plasmid and modulate placental transglutaminase activity. Furthermore, retinoids are secreted from JEG-3, as shown by the activation of retinoic acid-responsive ß lacZ reporter cells grown in conditioned media. These results suggest that there could be an active role for trophoblast-derived retinoids during human development.

development/ethanol/placenta/retinoic acid

6 To whom correspondence should be addressed at: INSERM U.384, Laboratoire de Biochimie Médicale, Faculté de Médecine et de Pharmacie, 28, Place Henri Dunant, BP.38, 63000 Clermont-Ferrand, France. E-mail: visapin{at}courrier.u-clermont1.fr


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