Molecular Human Reproduction, Vol. 8, No. 6, 511-517,
June 2002
© 2002 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays
1 Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, 2 Institute of Genetics, Chinese Academy of Sciences, Shanghai, 101300, 3 Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, P.R.China, 4 Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA, 5 Department of physiology, The Chinese University of Hong Kong, 2001 H.K. and 6 Institute of genetic resources, Nanjing Normal University, Nanjing, 210097, P.R.China
We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1–4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models.
cDNA array/development/spermatogenesis/testis
7 To whom correspondence should be addressed. E-mail: shajh{at}njmu.edu.cn
* Spermatogenesis study group, Hui Zhu1, Hu Zhu1, Yuxi Shan1, Min Lin1, Lirong Wang1, Lijun Cheng1, Yadong Zhou1and Yiquan Wang2
1 Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029,
2 Institute of Genetic Resources, Nanjing Normal University, Nanjing 210097, PR China
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