Molecular Human Reproduction, Vol. 8, No. 6, 531-539,
June 2002
© 2002 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Molecular cloning and characterization of the human orthologue of male germ cell-specific actin capping protein 
3 (cp3)
1 Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University and 2 Department of Urology, Osaka University Medical School, Osaka, Japan
We report here the molecular cloning and characterization of a novel human actin capping protein 
3 (cp3) cDNA, an orthologue of the mouse male germ cell-specific cp3, and the organization of the human cp3 genomic structure. The entire coding region of the human cp3 cDNA showed 82.1% similarity with the mouse cp3. The predicted amino acid sequence was 91.3% identical to the mouse protein and the actin-binding motif in the C-terminal region is highly conserved among species. The mRNA of the human cp3 gene was found to be exclusively expressed in the testis. Western blot analysis detected a 33 kDa protein in human testis and sperm. Immunohistochemistry showed that the main localization of human CP3 protein was in the neck region of ejaculated sperm, with moderate and faint signals also detected in the tail and postacrosome region respectively. Furthermore the localization of CP3 coincided with the species-specific distribution of actin in human sperm. The human cp3 gene was mapped to chromosome 12p12 by computer database cloning of human genomic DNA and was proven to be intronless. CP3 may play a physiologically important role in sperm architecture as well as in fertility of the human male.
actin/CP3/Gsg3/sperm/testis
3 To whom correspondence should be addressed at: Research Institute for Microbial Diseases, Osaka University, 3–1 Yamadaoka, Suita, Osaka 565-0871, Japan. E-mail: nishimun{at}biken.osaka-u.ac.jp
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