Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Magdinier, F.
Right arrow Articles by Dante, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Magdinier, F.
Right arrow Articles by Dante, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 8, No. 7, 630-635, July 2002
© 2002 European Society of Human Reproduction and Embryology

Embryology

Epigenetic marks at BRCA1 and p53 coding sequences in early human embryogenesis

Frédérique Magdinier1,*, Sandrine Giscard d'Estaing1,*, Claire Peinado1, Banu Demirci2, Cyril Berthet3, Jean Franciois Guérin2 and Robert Dante1,4

1 Laboratoire de Génétique, UMR 5641 CNRS, 2 Laboratoire de Biologie de la Reproduction et du Développement, UCBL1, 8 avenue Rockefeller and 3 INSERM U453, Centre Leon Berard, 69373 Lyon Cedex 08, France

In the vertebrate genome, methylation of deoxycytosine residues of CpGs dinucleotide has been associated with transcriptional silencing of genes, parental imprinting, X-inactivation and chromatin remodelling. In human somatic tissues, the 5' end of the BRCA1 CpG island is methylated, whereas this region is unmethylated in mature germ cells and early embryos. In gametes, as in somatic tissues, the CpG sites in the coding region are methylated. We took advantage of this bimodal distribution as a model to analyse the epigenetic reprogramming of coding regions during early human embryogenesis using the bisulphite-based genomic sequencing method. During preimplantation divisions, exon 11 of BRCA1 was slowly demethylated and retained ~30% of its methylated residues at the blastocyst stage. Moreover, the change in the distribution of methylated residues was not restricted to the BRCA1 gene, since for another gene, p53, a relatively high level of methylation (50%) of exon 4 was observed in blastocysts. Taken together, these data suggest that a significant part of the methylated residues of coding sequences might be conserved during preimplantation development.

bisulphite sequencing/CpG island/DNA methylation/human embryogenesis/single copy gene

4 To whom correspondence should be addressed. E-mail: dante{at}univ-lyon1.fr

* These authors have contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
M. M. Siddique, C. Balram, L. Fiszer-Maliszewska, A. Aggarwal, A. Tan, P. Tan, K. C. Soo, and K. Sabapathy
Evidence for Selective Expression of the p53 Codon 72 Polymorphs: Implications in Cancer Development
Cancer Epidemiol. Biomarkers Prev., September 1, 2005; 14(9): 2245 - 2252.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
H. Fulka, M. Mrazek, O. Tepla, and J. Fulka Jr
DNA methylation pattern in human zygotes and developing embryos
Reproduction, December 1, 2004; 128(6): 703 - 708.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.