Molecular Human Reproduction, Vol. 8, No. 7, 674-680,
July 2002
© 2002 European Society of Human Reproduction and Embryology
Implantation and pregnancy |
Microarray analysis of differentially expressed genes in placental tissue of pre-eclampsia: up-regulation of obesity-related genes
Department of Obstetrics and Gynaecology and Institute of Immunology, University of Rostock, Germany
Susceptibility genes present in both mother and fetus most likely contribute to the risk of pre-eclampsia. Placental biopsies were therefore investigated by high-density DNA microarray analysis to determine genes differentially regulated within chorionic villous tissue in pre-eclampsia and normal pregnancy. The pooled RNAs of pre-eclamptic and normotensive subjects were hybridized to the HuGeneFL array representing sequences from 
5600 full-length human cDNAs. The differentially expressed genes that were detected could be categorized into nine groups: adhesion molecules, obesity-related genes, transcription factors/signalling molecules, immunological factors, neuromediators, oncogenic factors, protease inhibitors, hormones and growth factor-binding proteins. Among those, the obesity-related genes included putative candidate genes associated with the pathogenesis of pre-eclampsia. One of the most up-regulated transcripts was the obese gene (43.6-fold change), and this was reflected by elevated leptin protein levels. In the case of feto-maternal contribution of polymorphic genes to pre-eclampsia, expression analysis of placental tissue has lead to numerous target genes waiting for large scale genetic linkage analyses.
leptin/microarray/obese gene/pre-eclampsia
1 To whom correspondence should be addressed at: Department of Obstetrics and Gynaecology, University of Rostock, P.O. Box 10 08 88, D-18055 Rostock, Germany. E-mail: toralf.reimer{at}med.uni-rostock.de
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