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Molecular Human Reproduction, Vol. 8, No. 8, 742-749, August 2002
© 2002 European Society of Human Reproduction and Embryology

Ovary and oogenesis

The significance of membrane type 1 metalloproteinase in structural involution of human corpora lutea

Kengo Manase1, Toshiaki Endo1,4, Hirofumi Henmi1, Yoshimitu Kitajima1, Kiyohiro Yamazaki1, Akira Nishikawa1, Toshihiro Mitaka2, Hiroshi Sato3 and Ryuichi Kudo1

1 Department of Obstetrics and Gynecology, 2 Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, South-1 West-16 Chuo-ku, Sapporo and 3 Department of Molecular Virology and Oncology, Kanazawa University, Takara-machi, Kanazawa, Ishikawa 920, Japan

The expression of membrane type 1 (MT1) matrix metalloproteinase (MMP), MMP-2, and tissue inhibitors of MMP-1 and -2 during structural involution of the human corpus luteum was examined using immunohistochemistry, Northern blotting, Western blotting, gelatin zymography and in-situ hybridization techniques. The corpora lutea of 20 patients were investigated at the time of total hysterectomy and were obtained from five patients each in the early, mid- and late luteal phases and during gestation. Immunohistochemistry for MT1-MMP in corpus luteum showed that the protein appeared in granulosa lutein cells in the late luteal phase. Both the expression of MT1-MMP mRNA and the amount of the protein increased in the late luteal phase. In-situ hybridization showed that MT1-MMP mRNA was localized mainly in the cytoplasm of granulosa lutein cells. These results suggest that increased expression of MT1-MMP may be a major factor in remodelling of the human corpus luteum during structural luteolysis.

human corpus luteum/MMP-2/MTI-MMP/TIMP-1/TIMP-2

4 To whom correspondence should be addressed. E-mail: endot{at}sapmed.ac.jp


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