Molecular Human Reproduction, Vol. 8, No. 8, 770-775,
August 2002
© 2002 European Society of Human Reproduction and Embryology
Uterine physiology |
Estrogen receptor-
and -ß expression in microvascular endothelial cells and smooth muscle cells of myometrium and leiomyoma
1 Centre for Women's Health Research, Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre and 2 Prince Henry's Institute of Medical Research 246 Clayton Road, Clayton, Victoria, 3168, Australia
The two estrogen receptors, ER
and ERß, are likely to have roles in the pathophysiology of fibroid development. They have been detected in myometrial and leiomyoma (fibroid) tissue, but the cell types expressing ER and ERß have not been determined. ERs have also been detected in human endothelial cells. The aims of the present study were to determine whether pure populations of myometrial microvascular endothelial cells (MEC) express ER and ERß, to compare MEC ER/ERß expression with that of pure populations of myometrial smooth muscle cells (SMC) and to determine if ER/ERß are differentially expressed in MEC and SMC of myometrium and fibroids from nine paired samples. Using RT–PCR (for ER and ERß) and Western blotting (for ER only), we demonstrated that all cultures of early passage myometrial and fibroid SMC (>99% pure) expressed ER but not ERß, while myometrial and fibroid MEC (>99% CD31+) constitutively expressed ERß. However, both myometrial and fibroid MEC showed variable expression of ER, with ~60% of MEC samples expressing ER. While the majority (6/9) of MEC from myometrial and fibroid pairs demonstrated the same pattern of ER expression, 3/9 pairs showed discordant ER expression. These results show that ER and ERß are differentially expressed in SMC and MEC of human myometrium and fibroids. Since ER and ERß mediate opposing transcriptional activities, any effect of estrogen on the growth and development of fibroids is likely to be complex and may involve both SMC and MEC.
ER/ERß/fibroid/myometrium/microvascular endothelial cells/smooth muscle cells
3 To whom correspondence should be addressed. E-mail: caroline.gargett{at}med.monash.edu.au
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