Molecular Human Reproduction, Vol. 8, No. 9, 811-816,
September 2002
© 2002 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Different signal transduction pathways are involved during human sperm capacitation induced by biological and pharmacological agents
1 Urology Research Laboratory, H6.44, Royal Victoria Hospital and McGill University, 687 Pine Avenue West, Montréal, Québec H3A 1A1, Canada
Human sperm capacitation involves complex signal transduction mechanisms during which double phosphorylation of the threonine-glutamine-tyrosine motif (P-Thr-Glu-Tyr-P) occurs in some sperm proteins. The objective of this study was to investigate the regulation of this process. Fetal cord serum ultrafiltrate (FCSu), follicular fluid ultrafiltrate (FFu), progesterone and a combination of N6,2'-O-dibutyryl cAMP (dbcAMP; cell permeant analogue of cAMP) and 3-isobutyl-1-methylxanthine (IBMX; phosphodiesterase inhibitor) were used as inducers of capacitation alone or in combination with inhibitors of protein kinase A (H89), protein kinase C (chelerythrine), protein tyrosine kinase (tyrphostin A47, PP2) and of dual specificity kinase (MEK-like kinases; PD98059). The level of P-Thr-Glu-Tyr-P in sperm proteins of 80 and 105 kDa during capacitation induced by FCSu, FFu and progesterone was regulated by a similar signal transduction pathway and involved receptor type protein tyrosine kinase and dual specificity kinase (MEK or MEK-like) but not protein kinase A or C. However, the level of P-Thr-Glu-Tyr-P in these sperm proteins during capacitation induced by dbcAMP+IBMX was mainly mediated through protein kinase A and C and receptor type protein tyrosine kinase, but not by dual specificity kinase. In conclusion, human sperm capacitation induced by some biological and pharmacological agents is regulated through very different signal transduction pathways.
dual specificity kinase/protein kinases/protein phosphorylation/sperm/Thr-Glu-Tyr phosphorylation
1 To whom correspondence should be addressed. E-mail: jthundathil{at}hotmail.com
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-F. Li, W. He, K. N. Jha, K. Klotz, Y.-H. Kim, A. Mandal, S. Pulido, L. Digilio, C. J. Flickinger, and J. C. Herr FSCB, a Novel Protein Kinase A-phosphorylated Calcium-binding Protein, Is a CABYR-binding Partner Involved in Late Steps of Fibrous Sheath Biogenesis J. Biol. Chem., November 23, 2007; 282(47): 34104 - 34119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. White, E. de Lamirande, and C. Gagnon Protein kinase C is an important signaling mediator associated with motility of intact sea urchin spermatozoa J. Exp. Biol., November 15, 2007; 210(22): 4053 - 4064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Bastian, A. Zepeda-Bastida, S. Uribe, and A. Mujica In spermatozoa, Stat1 is activated during capacitation and the acrosomal reaction Reproduction, September 1, 2007; 134(3): 425 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.N. Jha, A.M. Salicioni, E. Arcelay, O. Chertihin, S. Kumari, J.C. Herr, and P.E. Visconti Evidence for the involvement of proline-directed serine/threonine phosphorylation in sperm capacitation Mol. Hum. Reprod., December 1, 2006; 12(12): 781 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Baker, L. Hetherington, and R. J. Aitken Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa J. Cell Sci., August 1, 2006; 119(15): 3182 - 3192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. O'Flaherty, E. de Lamirande, and C. Gagnon Reactive Oxygen Species and Protein Kinases Modulate the Level of Phospho-MEK-Like Proteins During Human Sperm Capacitation Biol Reprod, July 1, 2005; 73(1): 94 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liguori, E. de Lamirande, A. Minelli, and C. Gagnon Various protein kinases regulate human sperm acrosome reaction and the associated phosphorylation of Tyr residues and of the Thr-Glu-Tyr motif Mol. Hum. Reprod., March 1, 2005; 11(3): 211 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Nauc, E. De Lamirande, P. Leclerc, and C. Gagnon Inhibitors of Phosphoinositide 3-Kinase, LY294002 and Wortmannin, Affect Sperm Capacitation and Associated Phosphorylation of Proteins Differently: Ca2+-Dependent Divergences J Androl, July 1, 2004; 25(4): 573 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R E Spindler, Y Huang, J G Howard, P Wang, H Zhang, G Zhang, and D E Wildt Acrosomal integrity and capacitation are not influenced by sperm cryopreservation in the giant panda Reproduction, May 1, 2004; 127(5): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O'Flaherty, E. de Lamirande, and C. Gagnon Phosphorylation of the Arginine-X-X-(Serine/Threonine) motif in human sperm proteins during capacitation: modulation and protein kinase A dependency Mol. Hum. Reprod., May 1, 2004; 10(5): 355 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Minelli, L. Liguori, I. Bellazza, R. Mannucci, B. Johansson, and B. B. Fredholm Involvement of A1 Adenosine Receptors in the Acquisition of Fertilizing Capacity J Androl, March 1, 2004; 25(2): 286 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Baker, L. Hetherington, H. Ecroyd, S. D. Roman, and R. J. Aitken Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation J. Cell Sci., January 15, 2004; 117(2): 211 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Kirkman-Brown Editorial Commentary J Androl, September 1, 2003; 24(5): 734 - 735. [Full Text] [PDF]
|
|