Molecular Human Reproduction, Vol. 8, No. 9, 855-863,
September 2002
© 2002 European Society of Human Reproduction and Embryology
Uterine physiology |
Microarray analysis of VEGF-responsive genes in myometrial endothelial cells
1 Centre for Women’s Health Research, Department of Obstetrics and Gynaecology, Monash University, 246 Clayton Rd, Clayton, Victoria 3168 and 2 Peter MacCallum Cancer Institute, Melbourne, Australia
There is evidence that the vasculature of different organs display different functional characteristics in response to cytokines and growth factors. The aim of this study was to use cDNA gene expression microarray to analyse changes in gene expression following stimulation of myometrial microvascular endothelial cells (MMECs) with vascular endothelial growth factor (VEGF). Primary isolates of MMECs were obtained from fresh hysterectomy specimens and purified with magnetic beads. Cells were stimulated with 15 ng/ml VEGF for 3, 6 and 12 h, and two unstimulated experiments served as controls. A total of six arrays was performed over these time-points. A total of 110 genes were identified as up-regulated by VEGF, 19% of which (21 genes) have previously been reported as up-regulated by VEGF or by angiogenesis. Among the novel genes to be up-regulated by VEGF were brain-derived growth factor, oxytocin receptor and estrogen sulphotransferase. The significance of the genes identified in the physiological and pathological functioning of the myometrial vasculature is discussed.
angiogenesis/endothelial cell/fibroids/myometrium/vascular endothelial growth factor (VEGF)
3 To whom correspondence should be addressed. E-mail: gareth.weston{at}med.monash.edu.au
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Zaitseva, B. J. Vollenhoven, and P. A.W. Rogers Retinoids regulate genes involved in retinoic acid synthesis and transport in human myometrial and fibroid smooth muscle cells Hum. Reprod., May 1, 2008; 23(5): 1076 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Aplin, M. Gelati, E. Fogel, E. Carnevale, and R. F. Nicosia Angiopoietin-1 and vascular endothelial growth factor induce expression of inflammatory cytokines before angiogenesis Physiol Genomics, October 3, 2006; 27(1): 20 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takahashi, L. Li, M. Kamiryo, T. Asteriou, A. Moustakas, H. Yamashita, and P. Heldin Hyaluronan Fragments Induce Endothelial Cell Differentiation in a CD44- and CXCL1/GRO1-dependent Manner J. Biol. Chem., June 24, 2005; 280(25): 24195 - 24204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bostrom, A. F. Zebboudj, Y. Yao, T. S. Lin, and A. Torres Matrix GLA Protein Stimulates VEGF Expression through Increased Transforming Growth Factor-{beta}1 Activity in Endothelial Cells J. Biol. Chem., December 17, 2004; 279(51): 52904 - 52913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Ponnampalam, G. C. Weston, A. C. Trajstman, B. Susil, and P. A.W. Rogers Molecular classification of human endometrial cycle stages by transcriptional profiling Mol. Hum. Reprod., December 1, 2004; 10(12): 879 - 893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Print, R. Valtola, A. Evans, K. Lessan, S. Malik, and S. Smith Soluble factors from human endometrium promote angiogenesis and regulate the endothelial cell transcriptome Hum. Reprod., October 1, 2004; 19(10): 2356 - 2366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Bond, L. J. Parry, C. S. Samuel, H. M. Gehring, F. L. Lederman, P. A. W. Rogers, and R. J. Summers Increased Expression of the Relaxin Receptor (LGR7) in Human Endometrium during the Secretory Phase of the Menstrual Cycle J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3477 - 3485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vignozzi, S. Filippi, M. Luconi, A. Morelli, R. Mancina, M. Marini, G. B. Vannelli, S. Granchi, C. Orlando, S. Gelmini, et al. Oxytocin Receptor Is Expressed in the Penis and Mediates an Estrogen-Dependent Smooth Muscle Contractility Endocrinology, April 1, 2004; 145(4): 1823 - 1834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Liu, H. Jia, D. I. R. Holmes, A. Stannard, and I. Zachary Vascular Endothelial Growth Factor-Regulated Gene Expression in Endothelial Cells: KDR-Mediated Induction of Egr3 and the Related Nuclear Receptors Nur77, Nurr1, and Nor1 Arterioscler. Thromb. Vasc. Biol., November 1, 2003; 23(11): 2002 - 2007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nakamura, Y. Miki, T. Suzuki, T. Nakata, A. D. Darnel, T. Moriya, C. Tazawa, H. Saito, T. Ishibashi, S. Takahashi, et al. Steroid Sulfatase and Estrogen Sulfotransferase in the Atherosclerotic Human Aorta Am. J. Pathol., October 1, 2003; 163(4): 1329 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Weston, A. C. Trajstman, C. E. Gargett, U. Manuelpillai, B. J. Vollenhoven, and P. A.W. Rogers Fibroids display an anti-angiogenic gene expression profile when compared with adjacent myometrium Mol. Hum. Reprod., September 1, 2003; 9(9): 541 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ho, E. Yang, G. Matcuk, D. Deng, N. Sampas, A. Tsalenko, R. Tabibiazar, Y. Zhang, M. Chen, S. Talbi, et al. Identification of endothelial cell genes by combined database mining and microarray analysis Physiol Genomics, May 13, 2003; 13(3): 249 - 262. [Abstract] [Full Text] [PDF]
|
|