Molecular Human Reproduction, Vol. 9, No. 1, 9-17,
January 2003
© 2003 European Society of Human Reproduction and Embryology
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Identification and characterization of a novel human testis-specific Golgi protein, NYD-SP12
Submitted on July 18, 2002; accepted on October 24, 2002
1 Laboratory of Reproductive Medicine, Center of Human Functional Genomics, Nanjing Medical University, Nanjing, 210029, People’s Republic of China and 2 Department of Life Sciences, Indiana State University, Terre Haute, IN 47809, USA 3 To whom correspondence should be addressed. e-mail: shajh{at}njmu.edu.cn
A novel human testis-specific gene, NYD-SP12, was identified by hybridizing human adult or fetal testes cDNA samples with a human cDNA microarray containing 9216 clones. mRNA expression level of NYD-SP12 was 30-fold higher in human adult testes than fetal testes. Similarly, semi-quantitative RT–PCR revealed a differential expression pattern of an NYD-SP12 homologous gene in mouse adult and infant testes. PCR and hybridization analysis of NYD-SP12 mRNA from multiple human tissues indicated the expression of NYD-SP12 exclusively in the testis. In-situ hybridization revealed that the expression of this gene was confined to spermatogenic epithelium and was not found in interstitial cells. NYD-SP12 transcript was not detected in patients with spermatogenic arrest and Sertoli cell-only syndrome. NYD-SP12 cDNA (GenBank accession number: AF345909) consisted of 2070 bp. The predicted 1707 bp open-reading fragment encoded a 569 amino acid protein that was 77% identical to a mouse homologue. Furthermore, computerized SMART and Motif analysis revealed that the protein contained a Structural Classification Of Proteins (SCOP) domain in the C-terminus and a cluster of phosphorylation sites for PKC, CK and cAMP/cGMP-dependent protein kinase. Interestingly, the EGFP-NYD-SP12 fusion protein was localized to the Golgi apparatus. In conclusion, the results suggest that NYD-SP12 is involved in spermatogenesis, and that NYD-SP12-encoded protein might function in the Golgi apparatus.
Key words: Golgi apparatus/infertility/NYD-SP12/spermatogenesis/testis
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