Molecular Human Reproduction, Vol. 9, No. 4, 213-218,
April 2003
© 2003 European Society of Human Reproduction and Embryology
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The effects of labour and of interleukin 1 beta upon the expression of nuclear factor kappa B related proteins in human amnion
Submitted on October 16, 2002; . accepted on December 16, 2002
1 Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, DuCane Road, London W12 0NN and 2 Biomedical Research Institute, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
3 To whom correspondence should be addressed. e-mail: pbennett{at}ic.ac.uk
Human labour is associated with persistently increased nuclear factor kappa B (NF-kB) activity in amnion. In this study we have shown that this involves only the p65 and p50 NF-kB subunits and is associated with an increase in the expression of p65 (P < 0.05), and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2 (P < 0.05). Interleukin-1b stimulation leads to rapid degradation and resynthesis of IkBa within 2 h, and a decrease in IkBb-1 without a return to full expression by 2 h, but has little effect upon IkBb-2. IkBb-2 was found in both the cytosolic and nuclear protein fractions. These findings demonstrate that persistently increased NF-kB activity in amnion occurs despite increased expression of the inhibitory IkBa protein and is not mediated by persistant I-kappa kinase activity or inhibition of IkBa synthesis. The increased expression and nuclear localization of IkBb-2 suggests that its function may be to protect NF-kB from inactivation by IkBa and to maintain NF-kB-mediated gene transcription.
Key words: amnion/interleukin /labour/NF-kB/parturition
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