Molecular Human Reproduction, Vol. 9, No. 5, 271-277,
May 2003
© 2003 European Society of Human Reproduction and Embryology
Article |
Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium
Submitted on October 17, 2002; accepted on February 8, 2003
1 Departments of Obstetrics & Gynecology and 2 Pathology, University Hospital, S-221 85 Lund, 3 Atherosclerosis Research Unit, King Gustav V Research Institute, Karolinska Hospital, S-171 76 Stockholm, Sweden and 4 Department of Obstetrics & Gynecology, Palacky University, 775 20 Olomouc, Czech Republic
5 To whom correspondence should be addressed. e-mail: bertil.casslen{at}gyn.lu.se
The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes.
Key words: c-jun/human endometrium/MMP-26/mRNA/proteinase/regulation
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