Molecular Human Reproduction, Vol. 9, No. 7, 429-435,
July 2003
© 2003 European Society of Human Reproduction and Embryology
Article |
Preimplantation genetic diagnosis for Charcot–Marie–Tooth disease type 1A
Submitted on February 8, 2003; accepted on March 27, 2003
1 Centre for Reproductive Medicine and 3 Centre for Medical Genetics, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Laarbeeklaan 101, B-1090 Brussels, Belgium
2 To whom correspondence should be addressed. e-mail: lriadvsa{at}az.vub.ac.be
Charcot–Marie–Tooth (CMT) disease is the ‘common’ name for a range of hereditary peripheral neuropathies. CMT1 is the most common form and is transmitted in an autosomal dominant manner. CMT1A maps to chromosome 17p11.2 and is caused, in the majority of cases, by a 1.5 Mb DNA duplication, that includes the peripheral myelin protein 22 (PMP) gene. This paper reports on preimplantation genetic diagnosis (PGD) for CMT1A in five couples. The CMT1A duplication was detected by fluorescent PCR analysis using polymorphic (CA)n markers localized within the duplication. Single-cell PCR on blastomeres allowed genetic analysis of embryos obtained after ICSI. Only healthy unaffected embryos were transferred to the uterus. PCR experiments with single EBV-transformed lymphoblasts or with research blastomeres allowed the evaluation of amplification efficiencies, as well as contamination and allele drop-out (ADO) rates for each PCR protocol. Three simplex PCR protocols (using one primer pair) and two duplex PCR protocols (using two primer pairs) were developed for CMT1A. Additionally, a protocol using all three primer pairs in triplex was also established. Thirteen clinical ICSI–PGD cycles were performed for five couples (12 simplex PCR cycles and one duplex PCR cycle), resulting in seven embryo transfers. Three singleton pregnancies ensued in two couples and three healthy babies were delivered. This report describes different fluorescent PCR-based tests which allow efficient and accurate single-cell level detection of the CMT1A duplication. On the basis of the presence of the healthy allele of the affected parent-to-be (and/or absence of the affected one), healthy embryos can be selected for transfer. The assays are suitable for PGD for other couples who present with the same CMT1A duplication [depending on their informativity for the (CA)n markers available] as described here.
Key words: Charcot–Marie–Tooth disease/preimplantation genetic diagnosis/single-cell PCR
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Goossens, M. De Rycke, A. De Vos, C. Staessen, A. Michiels, W. Verpoest, A. Van Steirteghem, C. Bertrand, I. Liebaers, P. Devroey, et al. Diagnostic efficiency, embryonic development and clinical outcome after the biopsy of one or two blastomeres for preimplantation genetic diagnosis Hum. Reprod., March 1, 2008; 23(3): 481 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pujol, J. Benet, C. Staessen, E. Van Assche, M. Campillo, J. Egozcue, and J. Navarro The importance of aneuploidy screening in reciprocal translocation carriers. Reproduction, June 1, 2006; 131(6): 1025 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Spits, M. De Rycke, N. Van Ranst, H. Joris, W. Verpoest, W. Lissens, P. Devroey, A. Van Steirteghem, I. Liebaers, and K. Sermon Preimplantation genetic diagnosis for neurofibromatosis type 1 Mol. Hum. Reprod., May 1, 2005; 11(5): 381 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De Rycke, I. Georgiou, K. Sermon, W. Lissens, P. Henderix, H. Joris, P. Platteau, A. Van Steirteghem, and I. Liebaers PGD for autosomal dominant polycystic kidney disease type 1 Mol. Hum. Reprod., January 1, 2005; 11(1): 65 - 71. [Abstract] [Full Text] [PDF]
|
|