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Mol. Hum. Reprod. Advance Access originally published online on December 5, 2005
Molecular Human Reproduction 2005 11(10):711-713; doi:10.1093/molehr/gah211
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Human villous trophoblast and the lack of intron 4-retaining soluble HLA-G secretion: beware of possible methodological biases

Philippe Le Bouteiller

INSERM U563, Hôpital Purpan, Toulouse, France

The first 150 words of the full text of this article appear below.


   Introduction
 
The straightforward Blaschitz et al. (2005)Go manuscript title makes the reader think that villous trophoblast cells in the human placenta definitely not secrete intron 4-retaining soluble HLA-G5 (also called soluble HLA-G1) molecules. Things might not be as simple... as the results presented by these authors contradict a number of previous reports on this subject (Chu et al., 1998Go; Solier et al., 2002Go; Ishitani et al., 2003Go; Morales et al., 2003Go). Rather than trying to resolve this issue, I like to focus on several experimental procedures and materials used by Blaschitz and collaborators and make the suggestion that some of them might have introduced possible biases that would possibly account for these discrepancies. Let us thus have a look at the materials and methods used by the authors.


   Types of cells
 
Besides placental tissue sections, the authors used several cell types to sustain their conclusions. . . . [Full Text of this Article]


   Real-time PCR
 

   Western blotting
 

   ELISA
 

   Immunohistochemistry
 

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A. van der Meer, H.G.M. Lukassen, B. van Cranenbroek, E.H. Weiss, D.D.M. Braat, M.J. van Lierop, and I. Joosten
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[Abstract] [Full Text] [PDF]