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Mol. Hum. Reprod. Advance Access originally published online on December 5, 2005
Molecular Human Reproduction 2005 11(10):715-717; doi:10.1093/molehr/gah223
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Soluble HLA-G isoforms: technical deficiencies lead to misinterpretations

Joan S. Hunt1,3 and Daniel E. Geraghty2

1University of Kansas Medical Center, Kansas City, KS and 2Fred Hutchinson Cancer Research Center, Seattle, WA, USA

3 To whom correspondence should be addressed at: Department of Anatomy and Cell Biology, University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, KS 66160, E-mail: jhunt@kumc.edu

The first 150 words of the full text of this article appear below.


   Introduction
 
The recent report authored by Blaschitz et al. (in press)Go would lead the reader to believe that human trophoblast cells cannot synthesize or secrete any of the soluble isoforms derived from splice variants of the HLA-G message. They propose that all of the soluble HLA-G presumed to be produced in trophoblast cells is, in fact, cleaved membrane HLA-G1. An interesting idea.

Yet several published works have presented solid, well documented evidence that argues against this position. The original article describing the molecular structure of soluble HLA-G (Fujii et al., 1994Go) showed that soluble protein encoded by the intron 4-containing mRNA could be clearly distinguished from membrane HLA-G1 by isoelectric focusing (IEF). Further, a class I protein (reactive with W6/32) with an IEF pattern completely overlapping with soluble HLA-G from transfected cells was demonstrated in JEG-3 choriocarcinoma cells. Also in that study, PCR primers specific for soluble HLA-G-encoding . . . [Full Text of this Article]


   Cell preparations
 

   Use of irrelevant cells
 

   Antibody specificity
 

   Reaching the maternal circulation
 

   Cell lineage and expression of HLA
 

   Copy number
 

   PCR experiments
 

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A. van der Meer, H.G.M. Lukassen, B. van Cranenbroek, E.H. Weiss, D.D.M. Braat, M.J. van Lierop, and I. Joosten
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