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Mol. Hum. Reprod. Advance Access originally published online on December 5, 2005
Molecular Human Reproduction 2005 11(10):723-727; doi:10.1093/molehr/gah238
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Soluble HLA-G, the discussion is going on!

A. Blaschitz1,*, H. Juch1,*, A. Volz2, H. Hutter1 and G. Dohr1,3

1Institute of Cell Biology, Histology and Embryology/Center of Molecular Medicine, Medical University of Graz, Graz, Austria and 2Institut für Immungenetik, Charité-Universitätsmedizin Berlin, Berlin, Germany

3 To whom correspondence should be addressed at: Institute of Cell Biology, Histology and Embryology, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/7, A-8010 Graz, Austria. E-mail: gottfried.dohr@meduni-graz.at

The first 150 words of the full text of this article appear below.


   Introduction
 
In 1986, Sir Karl Popper explained that refuting a given hypothesis is the only way to achieve progress in science (Popper, 1968Go). Hence we assume our well-controlled study on intron 4-containing isoforms of HLA-G in human placenta (Blaschitz et al., 2005, current issueGo) was important as it challenged a popular hypothesis and therefore has attracted great attention among the scientific HLA-G researching community.

We appreciate the initiation of a discussion forum, which was excellently introduced by R. Ivell (current issue). His editorial exactly represents our attitude towards scientific work in general and underlines some of the basic problems of biomedical research nowadays.

The very comprehensive review by Sargent (2005, current issue)Go summarizes debatable points in the topic of soluble HLA-G isoform expression and gives a proper overview also to those scientists who are less familiar with HLA-G.

To achieve agreement, special conferences on HLA-G, including pre-workshops, have . . . [Full Text of this Article]


   The dispute about the source of HLA-G5 secretion
 

   Comments on criticism of our methodology
 
Immunohistochemistry
Peptide control
Cell lines
Western blot
PCR experiments
ELISA

   Conclusions
 
Perspectives

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