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Mol. Hum. Reprod. Advance Access originally published online on September 28, 2006
Molecular Human Reproduction 2006 12(11):653; doi:10.1093/molehr/gal081
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Letter to the editor

What is the appropriate oxygen tension for in vitro culture?

Mark Sullivan1, Paula Galea and Sania Latif

Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Institute of Reproductive and Developmental Biology, Imperial College London, Hammersmith Hospital, London, UK

1 To whom correspondence should be addressed at: Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Institute of Reproductive and Developmental Biology, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK. E-mail: mark.sullivan@imperial.ac.uk

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Sir,

In vitro culture is generally performed at oxygen tensions that are close to atmospheric (~20%). Consideration of in vivo oxygen tensions suggests that 10–13% oxygen is the maximum that should be used, and for some tissues (e.g. placenta), lower levels are physiological.

The . . . [Full Text of this Article]


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