Erratum
for Lee et al., Mol. Hum. Reprod. 10 (4) 259-264.
Molecular Human Reproduction, Vol. 10, No. 7, pp. 553, 2004
Molecular Human Reproduction vol. 10 no. 7 © European Society of Human Reproduction and Embryology, 2004; all rights reserved
Developmental stage-specific expression of Rbm suggests its involvement in early phases of spermatogenesis
Jungmin Lee,
Jinpyo Hong,
Euisu Kim,
Kyungjin Kim,
Soo Woong Kim,
Hanumanthappa Krishnamurthy,
Sanny S.W. Chung,
Debra J. Wolgemuth and
Kunsoo Rhee
Mol. Hum. Reprod., 10, 259–264
The publisher wishes to apologise for the incorrect reproduction of Figure 2
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Figure 2 Rbm expression at the protein level. (A) Immunoblot analysis was carried out with four different mouse tissues, using the rabbit anti-mouse Rbm-specific polyclonal antibody generated. The sizes of the protein markers (Mrx10–3) are indicated on the left side of the figure. (B) Immunohistochemical analysis of Rbm was carried out with testicular sections from (a) adult, (b) 17 day old, (c) 9 day old, (d) 7 day old, and (e) at/at germ cell-deficient mice. In (f), the antigen (the GST-Rbm fusion protein, 1 µg protein per section) was added into the antibody reaction mixture. The DAB staining resulted in brownish Rbm-positive cells.
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Figure 3 Determination of developmental stages at which Rbm was expressed during spermatogenesis. (A and B) Immunohistochemical analysis of Rbm protein localization was carried out on testis from adult mice and the tubules were assessed as to the stage of the cycle of the seminiferous epithelium (Russell et al., 1990). The Roman numerals indicate the stage of the seminiferous tubule. (C) Summary of developmental stage-specific expression of Rbm in the mouse testis. Rbm was expressed in early stages of spermatogenesis, starting in spermatogonia. The Rbm protein was detected consistently in spermatogonia and preleptotene spermatocytes, and intermittently in leptotene spermatocytes. The Rbm was detected again at zygotene to early pachytene spermatocytes. No Rbm-specific signal was detected in pachytene spermatocytes at stage II-III onwards.
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